The effect of jasplakinolide on the thermodynamic properties of ADP.BeFx bound actin filaments

Roland Kardos, Andrea Vig, József Orbán, G. Hild, M. Nyitrai, D. Lőrinczy

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of BeFx and a natural toxin (jasplakinolide) was examined on the thermal stability of actin filaments by using differential scanning calorimetry. The phosphate analogue beryllium fluoride shifted the melting temperature of actin filaments (67.4 °C) to 83.7 °C indicating that the filaments were thermodynamically more stable in their complex with ADP.BeFx. A similar tendency was observed when the jasplakinolide was used in the absence of BeFx. When both the ADP.BeFx and the jasplakinolide bound to the actin filaments their collective effect was similar to that observed with ADP.BeFx or jasplakinolide alone. These results suggested that ADP.BeFx and jasplakinolide probably stabilize the actin filaments by similar molecular mechanisms.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalThermochimica Acta
Volume463
Issue number1-2
DOIs
Publication statusPublished - Oct 25 2007

Fingerprint

jasplakinolide
Administrative data processing
adenosine diphosphate
Adenosine Diphosphate
Actins
filaments
Thermodynamic properties
thermodynamic properties
beryllium fluorides
Beryllium
Melting point
Differential scanning calorimetry
Phosphates
Thermodynamic stability
phosphates
tendencies
thermal stability
heat measurement
melting
analogs

Keywords

  • α-Skeletal actin
  • ADP.BeF
  • DSC
  • Jasplakinolide
  • Thermal stability
  • Thermodynamics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemistry (miscellaneous)

Cite this

The effect of jasplakinolide on the thermodynamic properties of ADP.BeFx bound actin filaments. / Kardos, Roland; Vig, Andrea; Orbán, József; Hild, G.; Nyitrai, M.; Lőrinczy, D.

In: Thermochimica Acta, Vol. 463, No. 1-2, 25.10.2007, p. 77-80.

Research output: Contribution to journalArticle

@article{4d7985b4a15f49d28417542b5c50b9e7,
title = "The effect of jasplakinolide on the thermodynamic properties of ADP.BeFx bound actin filaments",
abstract = "The effect of BeFx and a natural toxin (jasplakinolide) was examined on the thermal stability of actin filaments by using differential scanning calorimetry. The phosphate analogue beryllium fluoride shifted the melting temperature of actin filaments (67.4 °C) to 83.7 °C indicating that the filaments were thermodynamically more stable in their complex with ADP.BeFx. A similar tendency was observed when the jasplakinolide was used in the absence of BeFx. When both the ADP.BeFx and the jasplakinolide bound to the actin filaments their collective effect was similar to that observed with ADP.BeFx or jasplakinolide alone. These results suggested that ADP.BeFx and jasplakinolide probably stabilize the actin filaments by similar molecular mechanisms.",
keywords = "α-Skeletal actin, ADP.BeF, DSC, Jasplakinolide, Thermal stability, Thermodynamics",
author = "Roland Kardos and Andrea Vig and J{\'o}zsef Orb{\'a}n and G. Hild and M. Nyitrai and D. Lőrinczy",
year = "2007",
month = "10",
day = "25",
doi = "10.1016/j.tca.2007.07.019",
language = "English",
volume = "463",
pages = "77--80",
journal = "Thermochimica Acta",
issn = "0040-6031",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - The effect of jasplakinolide on the thermodynamic properties of ADP.BeFx bound actin filaments

AU - Kardos, Roland

AU - Vig, Andrea

AU - Orbán, József

AU - Hild, G.

AU - Nyitrai, M.

AU - Lőrinczy, D.

PY - 2007/10/25

Y1 - 2007/10/25

N2 - The effect of BeFx and a natural toxin (jasplakinolide) was examined on the thermal stability of actin filaments by using differential scanning calorimetry. The phosphate analogue beryllium fluoride shifted the melting temperature of actin filaments (67.4 °C) to 83.7 °C indicating that the filaments were thermodynamically more stable in their complex with ADP.BeFx. A similar tendency was observed when the jasplakinolide was used in the absence of BeFx. When both the ADP.BeFx and the jasplakinolide bound to the actin filaments their collective effect was similar to that observed with ADP.BeFx or jasplakinolide alone. These results suggested that ADP.BeFx and jasplakinolide probably stabilize the actin filaments by similar molecular mechanisms.

AB - The effect of BeFx and a natural toxin (jasplakinolide) was examined on the thermal stability of actin filaments by using differential scanning calorimetry. The phosphate analogue beryllium fluoride shifted the melting temperature of actin filaments (67.4 °C) to 83.7 °C indicating that the filaments were thermodynamically more stable in their complex with ADP.BeFx. A similar tendency was observed when the jasplakinolide was used in the absence of BeFx. When both the ADP.BeFx and the jasplakinolide bound to the actin filaments their collective effect was similar to that observed with ADP.BeFx or jasplakinolide alone. These results suggested that ADP.BeFx and jasplakinolide probably stabilize the actin filaments by similar molecular mechanisms.

KW - α-Skeletal actin

KW - ADP.BeF

KW - DSC

KW - Jasplakinolide

KW - Thermal stability

KW - Thermodynamics

UR - http://www.scopus.com/inward/record.url?scp=34548698687&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548698687&partnerID=8YFLogxK

U2 - 10.1016/j.tca.2007.07.019

DO - 10.1016/j.tca.2007.07.019

M3 - Article

AN - SCOPUS:34548698687

VL - 463

SP - 77

EP - 80

JO - Thermochimica Acta

JF - Thermochimica Acta

SN - 0040-6031

IS - 1-2

ER -