The effect of ionizing radiation on the homeostasis and functional integrity of murine splenic regulatory T cells

Andrea Balogh, Eszter Persa, Eniko Noémi Bogdándi, Anett Benedek, H. Hegyesi, G. Sáfrány, Katalin Lumniczky

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Objective: Radiotherapy affects antitumor immune responses; therefore, it is important to study radiation effects on various compartments of the immune system. Here we report radiation effects on the homeostasis and function of regulatory T (Treg) cells, which are important in down-regulating antitumor immune responses. Methods: C57Bl/6 mice were irradiated with 2 Gy and alterations in splenic lymphocyte fractions analyzed at different intervals. Results: Total CD4+ numbers showed stronger decrease after irradiation than CD4+Foxp3+ Tregs. Tregs were less prone to radiation-induced apoptosis than CD4+Foxp3- T cells. The ratio of CD4+Foxp3- and CD4+Foxp3+ fractions within the proliferating CD4+ pool progressively changed from 74:26 in control animals to 59:41 eleven days after irradiation, demonstrating a more dynamic increase in the proliferation and regeneration of the Treg pool. The CD4+Foxp3+ fraction expressing cell-surface CTLA4, an antigen associated with Treg cell activation increased from 5.3 % in unirradiated mice to 10.5 % three days after irradiation. The expression of IL-10 mRNA was moderately upregulated, while TGF-β expression was not affected. On the other hand, irradiation reduced Treg capacity to suppress effector T cell proliferation by 2.5-fold. Conclusion: Tregs are more radioresistant, less prone to radiation-induced apoptosis, and have faster repopulation kinetics than CD4+Foxp3- cells, but irradiated Tregs are functionally compromised, having a reduced suppressive capacity.

Original languageEnglish
Pages (from-to)201-212
Number of pages12
JournalInflammation Research
Volume62
Issue number2
DOIs
Publication statusPublished - Feb 2013

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Regulatory T-Lymphocytes
Ionizing Radiation
Homeostasis
Radiation Effects
Radiation
Apoptosis
T-Lymphocytes
Surface Antigens
Interleukin-10
Regeneration
Immune System
Radiotherapy
Cell Proliferation
Lymphocytes
Messenger RNA

Keywords

  • Apoptosis
  • CTLA4
  • Irradiation
  • Ki67
  • Regulatory T cells

ASJC Scopus subject areas

  • Pharmacology
  • Immunology

Cite this

The effect of ionizing radiation on the homeostasis and functional integrity of murine splenic regulatory T cells. / Balogh, Andrea; Persa, Eszter; Bogdándi, Eniko Noémi; Benedek, Anett; Hegyesi, H.; Sáfrány, G.; Lumniczky, Katalin.

In: Inflammation Research, Vol. 62, No. 2, 02.2013, p. 201-212.

Research output: Contribution to journalArticle

Balogh, Andrea ; Persa, Eszter ; Bogdándi, Eniko Noémi ; Benedek, Anett ; Hegyesi, H. ; Sáfrány, G. ; Lumniczky, Katalin. / The effect of ionizing radiation on the homeostasis and functional integrity of murine splenic regulatory T cells. In: Inflammation Research. 2013 ; Vol. 62, No. 2. pp. 201-212.
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