The effect of inositol 1,4,5-trisphosphate and GTP on calcium release from rat liver microsomes

Gergely L. Lukács, György Hajnóczky, László Hunyady, András Spät

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and GTP mobilized 8% and 90% of the ionophore-releaseable Ca2+ pool from rat liver microsomes, respectively. In contrast to GTP, which acted after a lag-time, the Ins(1,4,5)P3-induced Ca2+ release was immediate. Poly(ethylene glycol) inhibited the effect of Ins(1,4,5)P3 and enhanced that of GTP. Ins(1,4,5)P3 accelerated and enhanced the GTP-induced Ca2+ release. Guanylyl imidodiphosphate inhibited competitively the GTP stimulated Ca2+ release, but not the GTP-dependent phosphorylation of the Mr 17 000 and 38 000 protein bands.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalBBA - Molecular Cell Research
Volume931
Issue number2
DOIs
Publication statusPublished - Nov 12 1987

Keywords

  • (Rat liver microsome)
  • Calcium ion release
  • GTP
  • Inositol trisphosphate
  • Protein phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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