The effect of hypothalamic lesions on hypothalamo-pituitary-adrenal axis activity and inflammation in adjuvant-induced arthritis

G. Makara, M. S. Harbuz, T. L. Coventry, D. S. Jessop, D. Zelena, S. L. Lightman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adjuvant-induced arthritis (AA) was induced in control and in hypothalamic lesioned Piebald-Viral-Glaxo (PVG) rats. Following discrete paraventricular nucleus (PVN) lesions plasma corticosterone was increased 14 days after adjuvant injection as in controls, when hind paw inflammation was apparent. PVN lesion did not affect the severity of inflammation. In contrast, following medial basal hypothalamus (MBH) lesions adjuvant did not increase corticosterone levels and the increase in paw volume at day 14 was potentiated. Basal proopiomelanocortin (POMC) mRNA expression in the anterior lobe was unchanged by PVN lesions and decreased by MBH lesions. AA increased POMC mRNA in controls and in both PVN and MBH lesioned rats. After complete MBH lesion, surviving anterior pituitary tissue maintained morning levels of corticosterone. Thus, AA may activate the hypothalamo-pituitary-adrenal axis without the mediation of PVN neurones projecting to the median eminence. However, the loss of the corticosterone response to AA and the increase in severity of inflammation in the MBH lesioned rats suggests a central (non-PVN) component mediates effects of inflammation. Furthermore, the increase in POMC mRNA in the MBH lesioned AA rats suggests that part of this process is not mediated by releasing factors in the hypothalamo-hypophysial portal system, and that extrahypothalamic (peripheral) mediators act on the pituitary during chronic inflammation.

Original languageEnglish
Pages (from-to)25-37
Number of pages13
JournalStress
Volume4
Issue number1
Publication statusPublished - 2001

Fingerprint

Middle Hypothalamus
Experimental Arthritis
Paraventricular Hypothalamic Nucleus
Corticosterone
Inflammation
Pro-Opiomelanocortin
Messenger RNA
Hypothalamo-Hypophyseal System
Portal System
Median Eminence
Neurons
Injections

Keywords

  • Adrenocorticotropic hormone (ACTH)
  • Corticosterone
  • In situ hybridization
  • Paraventricular nucleus
  • Prolactin
  • Proopiomelanocortin (POMC)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

The effect of hypothalamic lesions on hypothalamo-pituitary-adrenal axis activity and inflammation in adjuvant-induced arthritis. / Makara, G.; Harbuz, M. S.; Coventry, T. L.; Jessop, D. S.; Zelena, D.; Lightman, S. L.

In: Stress, Vol. 4, No. 1, 2001, p. 25-37.

Research output: Contribution to journalArticle

Makara, G. ; Harbuz, M. S. ; Coventry, T. L. ; Jessop, D. S. ; Zelena, D. ; Lightman, S. L. / The effect of hypothalamic lesions on hypothalamo-pituitary-adrenal axis activity and inflammation in adjuvant-induced arthritis. In: Stress. 2001 ; Vol. 4, No. 1. pp. 25-37.
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