The effect of heat-inactivated murine cytomegalovirus on host DNA synthesis of different cells

E. Gönczöl, J. Stone, J. M. Melero

Research output: Contribution to journalArticle

Abstract

Heat-inactivated murine cytomegalovirus (MCMV) stimulates cellular DNA synthesis in WME, NMG, 3T3, Wg1A, chick and NIL-8 cells, but active or u.v.-irradiated MCMV does not. The stimulation of DNA synthesis in NIL-8 and chick cells was studied in detail. We found that both the nuclear and the mitochondrial DNA synthesis were stimulated in these cells. There was no virus DNA synthesis during the period we studied (48 h). The stimulation of nuclear DNA synthesis was about threefold in NIL-8 and 2.5-fold in chick cells as measured by the rate of incorporation of 3H-thymidine (3H-dThd) in the CsCl fractions which banded at the density of cell DNA. The stimulation was about 9.5-fold in NIL-8 and 1.7-fold in chick cells as detected by autoradiography. There was a 3-fold an a 2.2-fold increase in the degree of incorporation of 3H-dThd into mitochondrial DNA of NIL-8 and chick cells, respectively. The amount of mitochondrial DNA obtained in infected cells of both kinds was about twice that in control cells. The synthesis of mitochondrial DNA was also stimulated by a factor of 2 in the thymidine kinaseless 3T3 cells which incorporate exogenous thymidine exclusively into mitochondrial DNA. There were no MCMV specific antigens detectable by immunofluorescence 5 h after infection, but diffuse nuclear fluorescence could be demonstrated 24 h after infection. Our results indicate that the heat-inactivated virus penetrates the cells, stimulates host DNA synthesis and induces synthesis of early MCMV antigens.

Original languageEnglish
Pages (from-to)415-426
Number of pages12
JournalJournal of General Virology
Volume39
Issue number3
Publication statusPublished - 1978

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Muromegalovirus
Hot Temperature
DNA
Mitochondrial DNA
Thymidine
3T3 Cells
DNA Viruses
Infection
Autoradiography
Fluorescent Antibody Technique
Cell Count
Fluorescence
Viruses
Antigens

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

The effect of heat-inactivated murine cytomegalovirus on host DNA synthesis of different cells. / Gönczöl, E.; Stone, J.; Melero, J. M.

In: Journal of General Virology, Vol. 39, No. 3, 1978, p. 415-426.

Research output: Contribution to journalArticle

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