The effect of fumonisins producing fusarium verticillioides on the microbiota in pig caecum

Huu Anh Dang, A. Zsolnai, Melinda Kovács, Brigitta Bóta, Gábor Mihucz, Roland Pósa, Kinga Marosi, Mariam Kachlek, Judit Szabó-Fodor

Research output: Contribution to journalArticle

Abstract

Fumonisin-producing fungal species, Fusarium verticillioides, culture was mixed in the diets of 6 piglets for 9 days (Fumonisin B1 [FB1] intake of 17 mg/kg) to investigate whether there is any potential alteration in the caecal bacterial communities between the experimental (with F. verticillioides) and control groups (without F. verticillioides). Plate count agar culturing technique was applied to measure the amount of aerobic and anaerobic bacteria, Escherichia coli, coliforms, Lactobacillus spp. and Clostridium perfringens. A significant difference was observed between the control and experimental group only in the case of aerobic bacteria on Day 4, 8.60 ± 0.22 compared to 8.06 ± 0.20 (P < 0.05), respectively. Quantitative polymerase chain reaction (qPCR) was performed to estimate the DNA copy number of total bacteria, Bacteroides and Prevotella spp., Clostridium spp., E. coli, Enterobacteriales, Firmicutes and Lactobacillus spp. Significant differences were observed between the control and experimental group regarding total bacteria on Day 2 and Day 6, Firmicutes on Day 2 and E. coli and Enterobacteriales on Day 4. Regarding the entire feeding time, no significant difference between the two groups was found in all species of investigated bacteria by the culturing technique and qPCR after an 8-day exposure. The present research contributes to the understanding of how microbiota responds to the FB1 load.

Original languageEnglish
Pages (from-to)65-71
Number of pages7
JournalActa Veterinaria Brno
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Fumonisins
Microbiota
fumonisins
Fusarium
cecum
Aerobic Bacteria
Swine
Lactobacillus
Escherichia coli
Bacteria
Enterobacteriales
Control Groups
swine
bacteria
fumonisin B1
Firmicutes
Prevotella
Polymerase Chain Reaction
Clostridium perfringens
Bacteroides

Keywords

  • Caecal
  • Culturing
  • Fumonisin B1
  • QPCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

The effect of fumonisins producing fusarium verticillioides on the microbiota in pig caecum. / Dang, Huu Anh; Zsolnai, A.; Kovács, Melinda; Bóta, Brigitta; Mihucz, Gábor; Pósa, Roland; Marosi, Kinga; Kachlek, Mariam; Szabó-Fodor, Judit.

In: Acta Veterinaria Brno, Vol. 88, No. 1, 01.01.2019, p. 65-71.

Research output: Contribution to journalArticle

Dang, HA, Zsolnai, A, Kovács, M, Bóta, B, Mihucz, G, Pósa, R, Marosi, K, Kachlek, M & Szabó-Fodor, J 2019, 'The effect of fumonisins producing fusarium verticillioides on the microbiota in pig caecum', Acta Veterinaria Brno, vol. 88, no. 1, pp. 65-71. https://doi.org/10.2754/avb201988010065
Dang, Huu Anh ; Zsolnai, A. ; Kovács, Melinda ; Bóta, Brigitta ; Mihucz, Gábor ; Pósa, Roland ; Marosi, Kinga ; Kachlek, Mariam ; Szabó-Fodor, Judit. / The effect of fumonisins producing fusarium verticillioides on the microbiota in pig caecum. In: Acta Veterinaria Brno. 2019 ; Vol. 88, No. 1. pp. 65-71.
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