The effect of fat rich diet and alcohol on ion concentration in bile fluid in rats

Research output: Contribution to journalArticle

16 Citations (Scopus)


The metabolic alteration of fatty liver causes significant changes in the composition of bile fluid secreted by epithelial liver cells. These processes include both organic molecular and inorganic ion content changes in bile juice as well. In this work ion concentration differences of bile fluid in experimental hyperlipidemic rats compared to normal ones were monitored. Fatty liver in young male Wistar albino rats was induced by 2% cholesterol, 20% sunflower oil and 0.5% cholic acid added to the normal food and 3% alcoholic water was administered to rats for 9 days. The development of hyperlipidemy was detected by measuring serum parameters and fatty liver was proved by morphological investigations. The ion concentrations of bile fluid samples were determined by ICP-OES (inductively coupled plasma optical emission spectrometry). The bile fluid samples were digested with a mixture of HNO3 and H2O2. The results show that the concentration of calcium and phosphorus in bile juice increased significantly in hyperlipidemic rats compared to the control. Significant concentration changes have also been detected for chromium and manganese. The changes in metal ion metabolism also point to the damage of liver cells owing to metal complex evacuation.

Original languageEnglish
Pages (from-to)359-366
Number of pages8
JournalActa Alimentaria
Issue number4
Publication statusPublished - Dec 2000


  • Fatty liver
  • Metal-
  • Phosphorus- and sulfur concentration

ASJC Scopus subject areas

  • Food Science

Fingerprint Dive into the research topics of 'The effect of fat rich diet and alcohol on ion concentration in bile fluid in rats'. Together they form a unique fingerprint.

  • Cite this