The effect of estrogens on superoxide anion generation by human neutrophil granulocytes: Possible consequences of the antioxidant defense

Gabor Bekesi, Z. Tulassay, K. Rácz, J. Fehér, B. Székács, Reka Kakucs, E. Dinya, Eva Riss, Zoltan Magyar, J. Rigó

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The present study aimed to test whether, beyond the known antioxidant effect of estradiol, such a property is also possessed by estrone and estriol. For this purpose, an in vitro investigation of the effect of estrone and estriol on superoxide anion production by human neutrophil granulocytes was carried out. Blood samples were obtained from healthy volunteers and neutrophil granulocytes were separated for measurement of superoxide anion generation after incubation with estrone, estriol (10-7, 10-6 and 10-5 M) and 17β-estradiol (10-7 M). Superoxide anion production of isolated neutrophil granulocytes was quantified by photometry and using the reduction of ferricytochrome-C. When adding estrone and estriol to neutrophil granulocyte suspensions, the production of superoxide anion fell (10-5 M: 84.17 ± 3.14% and 88.77 ± 1.98% of control production, p <0.01 and p <0.05, respectively). Estradiol produced an antioxidant effect at lower concentration (10-7 M: 72.91 ± 7.94% of control production, p <0.001). The weak estrogens estrone and estriol, similarly to estradiol, are also able to reduce the superoxide anion release in our experimental model. This may have importance in the antioxidant defense of biological systems.

Original languageEnglish
Pages (from-to)451-454
Number of pages4
JournalGynecological Endocrinology
Volume23
Issue number8
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Estriol
Estrone
Granulocytes
Superoxides
Estrogens
Neutrophils
Antioxidants
Estradiol
Photometry
Suspensions
Healthy Volunteers
Theoretical Models

Keywords

  • Antioxidant effect
  • Estradiol
  • Pre-eclampsia
  • Superoxide anion
  • Weak estrogens

ASJC Scopus subject areas

  • Endocrinology
  • Obstetrics and Gynaecology

Cite this

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abstract = "The present study aimed to test whether, beyond the known antioxidant effect of estradiol, such a property is also possessed by estrone and estriol. For this purpose, an in vitro investigation of the effect of estrone and estriol on superoxide anion production by human neutrophil granulocytes was carried out. Blood samples were obtained from healthy volunteers and neutrophil granulocytes were separated for measurement of superoxide anion generation after incubation with estrone, estriol (10-7, 10-6 and 10-5 M) and 17β-estradiol (10-7 M). Superoxide anion production of isolated neutrophil granulocytes was quantified by photometry and using the reduction of ferricytochrome-C. When adding estrone and estriol to neutrophil granulocyte suspensions, the production of superoxide anion fell (10-5 M: 84.17 ± 3.14{\%} and 88.77 ± 1.98{\%} of control production, p <0.01 and p <0.05, respectively). Estradiol produced an antioxidant effect at lower concentration (10-7 M: 72.91 ± 7.94{\%} of control production, p <0.001). The weak estrogens estrone and estriol, similarly to estradiol, are also able to reduce the superoxide anion release in our experimental model. This may have importance in the antioxidant defense of biological systems.",
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AU - Bekesi, Gabor

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AU - Fehér, J.

AU - Székács, B.

AU - Kakucs, Reka

AU - Dinya, E.

AU - Riss, Eva

AU - Magyar, Zoltan

AU - Rigó, J.

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