The effect of endogenous angiotensin on the biosynthesis of aldosterone in the rat

A. Spät, T. Balla, P. Enyedi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Modification of the site of action of angiotensin II in adrenal cortex by the duration of stimulation and by changes in salt-water balance was examined in three different forms of sodium depletion. Increased formation of angiotensin was induced in conscious rats by peritoneal dialysis with glucose solution, administration of the diuretic drug furosemide or dietary sodium restriction. The conversion of corticosterone to aldosterone by incubated adrenal capsular tissue was estimated by conversion techniques using tracer amounts and excess amounts of substrate. Acute peritoneal or diuretic sodium depletion failed to affect the conversion rate of tracer amount of [3H]-corticosterone to [3H]-aldosterone but decreased the specific activity of [3H]-aldosterone. Dietary sodium restriction increased, peritoneal dialysis did not change and administration of furosemide decreased the formation of aldosterone from excess amounts of exogenous corticosterone in the presence of cyanoketone. These results, together with the results of our previous superfusion experiments, suggest that increased conversion of corticosterone to aldosterone is a phenomenon specific for chronic sodium depletion. In acute sodium deficiency the action of angiotensin II is confined to a step before the formation of corticosterone. The importance of discrepancies between tracer and excess substrate conversion experiments is discussed.

Original languageEnglish
Pages (from-to)1147-1152
Number of pages6
JournalJournal of Steroid Biochemistry
Volume14
Issue number11
DOIs
Publication statusPublished - 1981

Fingerprint

Biosynthesis
Angiotensins
Corticosterone
Aldosterone
Rats
Sodium
Dietary Sodium
Furosemide
Peritoneal Dialysis
Dialysis
Diuretics
Angiotensin II
Cyanoketone
Adrenal Cortex
Saline water
Substrates
Salts
Glucose
Experiments
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

The effect of endogenous angiotensin on the biosynthesis of aldosterone in the rat. / Spät, A.; Balla, T.; Enyedi, P.

In: Journal of Steroid Biochemistry, Vol. 14, No. 11, 1981, p. 1147-1152.

Research output: Contribution to journalArticle

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