The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the Crassulacean acid metabolism plant Kalanchoe daigremontiana

Mark R. Truesdale, Otto Toldi, Peter Scott

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In C3 plants, the metabolite fructose 2,6-bisphosphate (Fru 2,6- P2) has an important role in the regulation of carbon partitioning during photosynthesis. To investigate the impact of Fru 2,6-P2 on carbon metabolism during Crassulacean acid metabolism (CAM), we have developed an Agrobacterium tumefaciens-mediated transformation system in order to alter genetically the obligate CAM plant Kalanchoe daigremontiana. To our knowledge, this is the first report to use genetic manipulation of a CAM species to increase our understanding of this important form of plant metabolism. Transgenic plants were generated containing a modified rat liver 6-phosphofructo-2-kinase gene. In the plants analyzed the activity of 6-phosphofructo-2-kinase ranged from 175% to 198% of that observed in wild-type plants, resulting in Fru 2,6-P2 concentrations that were 228% to 350% of wild-type plants after 2 h of illumination. A range of metabolic measurements were made on these transgenic plants to investigate the possible roles of Fru 2,6-P2 during Suc, starch, and malic acid metabolism across the deacidification period of CAM. The results suggest that Fru 2,6-P2 plays a major role in regulating partitioning between Suc and starch synthesis during photosynthesis. However, alterations in Fru 2,6-P2 levels had little effect on malate mobilization during CAM fluxes.

Original languageEnglish
Pages (from-to)957-964
Number of pages8
JournalPlant physiology
Volume121
Issue number3
DOIs
Publication statusPublished - Nov 1999

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Fingerprint Dive into the research topics of 'The effect of elevated concentrations of fructose 2,6-bisphosphate on carbon metabolism during deacidification in the Crassulacean acid metabolism plant Kalanchoe daigremontiana'. Together they form a unique fingerprint.

  • Cite this