The effect of electroporation of a lyotroic liquid crystal genistein-based formulation in the recovery of murine melanoma lesions

Corina Danciu, Szilvia Berkó, Gábor Varju, Boglárka Balázs, Lajos Kemény, István Balázs Németh, Andreea Cioca, Alexandra Petruș, Cristina Dehelean, Citu Ioan Cosmin, Elena Amaricai, Claudia Crina Toma

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Abstract

A lamellar lyotropic liquid crystal genistein-based formulation (LLC-Gen) was prepared in order to increase the aqueous solubility of the lipophilic phytocompound genistein. The formulation was applied locally, in a murine model of melanoma, with or without electroporation. The results demonstrated that, when the formulation was applied by electroporation, the tumors appeared later. During the 21 days of the experiment, the LLC-Gen formulation decreased the tumor volume, the amount of melanin and the degree of erythema, but when electroporation was applied, all these parameters indicated a better prognosis even (lower tumor volume, amount of melanin and degree of erythema). Although hematoxylin–eosin (HE) staining confirmed the above events, application of the LLC-Gen formulation by electroporation did not lead to a significant effect in terms of the serum concentrations of the protein S100B and serum neuron specific enolase (NSE), or the tissue expression of the platelet-derived growth factor receptor β (PDGFRβ) antibody.

Original languageEnglish
Pages (from-to)15425-15441
Number of pages17
JournalInternational journal of molecular sciences
Volume16
Issue number7
DOIs
Publication statusPublished - Jul 8 2015

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Keywords

  • Electroporation
  • Genistein
  • Lyotropic liquid crystals
  • Murine melanoma

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Danciu, C., Berkó, S., Varju, G., Balázs, B., Kemény, L., Németh, I. B., Cioca, A., Petruș, A., Dehelean, C., Cosmin, C. I., Amaricai, E., & Toma, C. C. (2015). The effect of electroporation of a lyotroic liquid crystal genistein-based formulation in the recovery of murine melanoma lesions. International journal of molecular sciences, 16(7), 15425-15441. https://doi.org/10.3390/ijms160715425