The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary

A. Horváth, György Hoitsy, Balázs Kovács, Dóra Kánainé Sipos, Ágnes Osz, Klavdija Bogataj, B. Urbányi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Molecular markers (PCR-RFLP and microsatellite) were used to investigate the genetic background of the only brown trout (Salmo trutta m fario) broodstock in Hungary which due to the hydrogeography of the country should theoretically belong to the Danubian lineage. PCR-RFLP (mitochondrial DNA control region and lactate dehydrogenase and somatolactin genes) as well as microsatellite (BFRO002, OMM1064, Ssa408uos, SsoSL417, SsoSL438) markers were used to distinguish between Danubian and Atlantic lineages of brown trout in the Lillafüred broodstock. Altogether 435 fish were tagged during the experimental period. Due to mortalities, fin clips were collected from 401 individuals. According to the genetic analysis of the mitochondrial DNA, the Danubian haplotype is present in only one individual (0.2 %) of the broodstock. Analysis of the nuclear markers revealed that alleles characteristic of both the Atlantic and the Danubian lineages are found in the population. However, Atlantic alleles dominate throughout the broodstock which is in Hardy-Weinberg equilibrium according to the investigated markers. Results indicate that the original broodstock that was introduced to the farm following its construction in 1933 was of the Atlantic lineage. Although later fish from a local stream were collected and added to the broodstock, the number of these was limited and they were almost exclusively males. Fish from this farm that are stocked by anglers can have a significant genetic impact on trout populations of natural streams.

Original languageEnglish
Pages (from-to)5-11
Number of pages7
JournalAquaculture International
Volume22
Issue number1
DOIs
Publication statusPublished - Feb 2014

Fingerprint

breeding stock
domestication
Salmo trutta
Hungary
mitochondrial DNA
allele
fish
farm
restriction fragment length polymorphism
genetic analysis
microsatellite repeats
alleles
farms
lactate dehydrogenase
mortality
trout
genetic background
fins
genetic techniques and protocols
marker

Keywords

  • Brown trout
  • Genetics
  • Population structure
  • Stocking

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Aquatic Science

Cite this

The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary. / Horváth, A.; Hoitsy, György; Kovács, Balázs; Kánainé Sipos, Dóra; Osz, Ágnes; Bogataj, Klavdija; Urbányi, B.

In: Aquaculture International, Vol. 22, No. 1, 02.2014, p. 5-11.

Research output: Contribution to journalArticle

Horváth, A. ; Hoitsy, György ; Kovács, Balázs ; Kánainé Sipos, Dóra ; Osz, Ágnes ; Bogataj, Klavdija ; Urbányi, B. / The effect of domestication on a brown trout (Salmo trutta m fario) broodstock in Hungary. In: Aquaculture International. 2014 ; Vol. 22, No. 1. pp. 5-11.
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