The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus Carpio) to the white spot parasite, Ichthyophthirius Multifiliis

Dávid Herczeg, Dóra Sipos, A. Dán, Christina Loy, Dennis M. Kallert, E. Eszterbauer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered β-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% β-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-β (IL-1-β) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the β-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-β significantly decreased in the propolis-fed group at day 10. In the β-glucan-fed group, a significant IL-1-β decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-β was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.

Original languageEnglish
Pages (from-to)517-530
Number of pages14
JournalActa Veterinaria Hungarica
Volume65
Issue number4
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Ichthyophthirius multifiliis
Propolis
Immunologic Adjuvants
immunostimulants
propolis
Glucans
Carps
interleukin-1
Dental Caries
glucans
Interleukin-1
Cyprinus carpio
Parasites
parasites
Infection Control
disease control
Fishes
freshwater aquaculture
Aquaculture
liver

Keywords

  • Common carp
  • Ich
  • Immunostimulants
  • Propolis extract E50
  • White spot disease
  • β-glucan

ASJC Scopus subject areas

  • veterinary(all)

Cite this

The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus Carpio) to the white spot parasite, Ichthyophthirius Multifiliis. / Herczeg, Dávid; Sipos, Dóra; Dán, A.; Loy, Christina; Kallert, Dennis M.; Eszterbauer, E.

In: Acta Veterinaria Hungarica, Vol. 65, No. 4, 01.12.2017, p. 517-530.

Research output: Contribution to journalArticle

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