Cytomegalovirus (CMV) induced intracellular antigens have a regular sequence of development. In the presence of cytosine arabinoside (ara C) antigens only appeared in the form of diffusely distributed small granules in the nucleus; however, ultrasonically disrupted cells yielded no detectable infectious virus. The appearance of cytopathic changes depended on the amount of virus used for inoculation. With an inoculum of 3 to 5 TCD50/cell, de novo DNA synthesis began at 12-24 hr after infection and even continued beyond 36 hr. In the presence of ara C added in the course of DNA synthesis, the existing viral genomes developed into mature virions and all the antigen components appeared.
|Number of pages||7|
|Publication status||Published - Dec 1 1973|
ASJC Scopus subject areas
- Infectious Diseases