The effect of bradykinin and substance P on the arachidonate cascade of platelets

Árpád Gecse, Béla Kis, Zsófia Mezei, Gyula Telegdy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Several peptide receptors are expressed on the surface of platelets, including B1 and NK1, through which Bk and SP might influence platelet functions including their arachidonate cascade. The metabolites of the arachidonate cascade might play a regulatory role in the inter- and intracellular functions of platelets. Platelets were separated from fresh rat blood by differential centrifugation. Platelets (108 ml-1 in each sample) were preincubated with Bk or SP. The arachidonate cascade was investigated with [1-14C]arachidonic acid, as tracer substrate. The synthesised [14C]eicosanoids were isolated and quantitatively determined. Bradykinin elicited a biphasic dose-response curve in the formation of the vasoconstrictor and platelet aggregating thromboxane A2 (TxA2). Bk both inhibited (10-8 mol/l), and elevated (10-6 mol/l) the synthesis of TxA2 in the thrombocytes. The 12-HETE synthesis was inhibited by Bk (10-8, 10-7, 10-5 mol/l); 12-HETE is an endogenous regulator of prostacyclin synthesis. The formation of 12-HETE in platelets was stimulated by SP (10-11, 10-9, 10-8 mol/l). The synthesis of TxA2 in platelets was either attenuated (10-12 mol/l), or stimulated (10-9 mol/l) by SP. According to our observations Bk and SP might play a regulatory role in the activation or deactivation of platelets.

Original languageEnglish
Pages (from-to)167-170
Number of pages4
JournalImmunopharmacology
Volume33
Issue number1-3
DOIs
Publication statusPublished - Jun 1996

Keywords

  • 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE)
  • bradykinin
  • platelet
  • substance P
  • thromboxane

ASJC Scopus subject areas

  • Pharmacology

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