The effect of arbutin on membrane integrity during drying is mediated by stabilization of the lamellar phase in the presence of nonbilayer-forming lipids

Ann E. Oliver, Dirk K. Hincha, Nelly M. Tsvetkova, Laszlo Vigh, John H. Crowe

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Arbutin (4-hydroxyphenyl-β-glucopyranoside) is a solute accumulated to high concentrations in drought and frost resistant plants. Arbutin can inhibit membrane lysis, both free radical-mediated and enzymatic in nature, and it has been suggested that arbutin might contribute to membrane stabilization in these plants. However, we found that arbutin destabilized phosphatidylcholine vesicles during drying and rehydration, which appears to be inconsistent with the proposed protective function of arbutin for membranes. We also found, however, that arbutin stabilizes membranes containing nonbilayer-forming lipids during freezing. We now report that, in liposomes containing the nonbilayer-forming lipids monogalactosyldiacylglycerol (MGDG) or phosphatidylethanolamine (PE), arbutin served a protective function during drying, as measured by retention of carboxyfluorescein (CF) and extent of vesicle fusion. In hydrated samples containing these lipids, arbutin stabilized the lamellar liquid crystalline phase. Therefore, the interaction between arbutin and lipid membranes and the resulting effects on membrane stability depend, in a complex manner, on the lipid composition of the membrane.

Original languageEnglish
Pages (from-to)37-57
Number of pages21
JournalChemistry and Physics of Lipids
Volume111
Issue number1
DOIs
Publication statusPublished - Jul 11 2001

Keywords

  • Arbutin
  • Dehydration
  • Desiccation tolerance
  • Hexagonal II phase
  • Monogalactosyldiacylglycerol
  • Phosphatidylethanolamine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

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