The effect of angiotensin II on arachidonate metabolism in adrenal glomerulosa cells

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Abstract

The effect of angiotensin II on arachidonate metabolism was examined in rat adrenal glomerulosa cells. Incorporation of both [3H]arachidonate and [32P]phosphate into phosphatidylinositol (PI) were significantly stimulated by angiotensin II. These effects were abolished by lithium, a cation, which was found suitable to prevent increased synthesis of PI in our previous study (T. Balla et al., FEBS Letters 171, 179, 1984). On the other hand, the phospholipase A2 inhibitor mepacrine failed to inhibit the increased labelling of PI. These observations suggest that the increased 3H labelling of PI occurs via CDP-diacylglycerol, and not via enhanced deacylation-reacylation cycle. The validity of this assumption was further supported, since angiotensin II failed to stimulate the formation of lyso-PI, as examined by both [32P]phosphate incorporation and pulse-chase techniques. Angiotensin II decreased the incorporation of [3H]arachidonate into phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Considering that we did not find arachidonate release either from phospholipids or from other possible arachidonate sources this decrease may not be due to dilution of the tracer. Thus we assume that angiotensin II may induce a shift in phospholipid synthesis from PC and PE to phosphoinositides. These observations indicate that the enhanced hydrolysis and synthesis of PI in response to angiotensin II is not associated with increased phospholipase A2 activity in adrenal glomerulosa cells.

Original languageEnglish
Pages (from-to)3439-3444
Number of pages6
JournalBiochemical Pharmacology
Volume34
Issue number19
DOIs
Publication statusPublished - Oct 1 1985

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Zona Glomerulosa
Phosphatidylinositols
Metabolism
Angiotensin II
Phosphatidylcholines
Phospholipids
Cytidine Diphosphate Diglycerides
Labeling
Phosphatidylinositol Phosphates
Quinacrine
Phosphates
Phospholipases A2
Lithium
Cations
Hydrolysis
Dilution
Rats

ASJC Scopus subject areas

  • Pharmacology

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The effect of angiotensin II on arachidonate metabolism in adrenal glomerulosa cells. / Hunyady, László; Balla, Tamás; Enyedi, Péter; Spät, András.

In: Biochemical Pharmacology, Vol. 34, No. 19, 01.10.1985, p. 3439-3444.

Research output: Contribution to journalArticle

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