The effect of 4-aminopyridine on acetylcholine release

E. Vízi, J. van Dijk, F. F. Foldes

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The effect of 2-, and 4-aminopyridine (4-APYR) on the release mechanism of acetylcholine (ACh) from the nerve terminals of the Auerbach plexus-longitudinal muscle preparation of the guinea-pig ileum, suspended in eserinized Krebs' solution, was investigated. 2- and 4-APYR increased the release of ACh from the nerve terminals at rest and at both low and high frequency stimulation. The enhanced ACh release was found to be due to increased volley output. At lower frequency of stimulation, the potentiation of ACh release was much higher than at higher rate of stimulation. 4-APYR was able to increase ACh release in the absence of [Ca2+]0. However, when a Ca-chelating agent, EDTA, was also added to the Ca-free Krebs' solution, 4-APYR was entirely ineffective. The depression of ACh release induced by Mg-excess was completely antagonized by 4-APYR. Tetrodotoxin (TTX) prevented augmentation of ACh release by 4-APYR. It is suggested that 4-APYR lowers the demand of nerve terminals for [Ca2+]0 required for the excitation-secretion coupling process. The presence of a low concentration [Ca2+]0, however, is essential for the action of 4-APYR.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalJournal of Neural Transmission
Volume41
Issue number4
DOIs
Publication statusPublished - Dec 1977

Fingerprint

4-Aminopyridine
Acetylcholine
Myenteric Plexus
Secretory Pathway
Tetrodotoxin
Chelating Agents
Ileum
Edetic Acid
Guinea Pigs
Muscles

Keywords

  • 4-aminopyridine
  • ACh release
  • Ca requirement
  • Mg excess
  • nerve terminals

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

The effect of 4-aminopyridine on acetylcholine release. / Vízi, E.; van Dijk, J.; Foldes, F. F.

In: Journal of Neural Transmission, Vol. 41, No. 4, 12.1977, p. 265-274.

Research output: Contribution to journalArticle

Vízi, E. ; van Dijk, J. ; Foldes, F. F. / The effect of 4-aminopyridine on acetylcholine release. In: Journal of Neural Transmission. 1977 ; Vol. 41, No. 4. pp. 265-274.
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