The effect of 1-MCP on the expression of several ripening-related genes in strawberries

Andrea Balogh, Tímea Koncz, Viktória Tisza, E. Kiss, L. Heszky

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To elucidate the role of ethylene in nonclimacteric fruit development and ripening, quantitative (cDNA-amplified fragment length polymorphism) cDNA-AFLP was used to visualize differential gene expression in four stages of ripening of strawberries (Fragaria xananassa Duch. 'Elsanta') treated with 1-methylcyclopropene (1-MCP), a competitive inhibitor of ethylene action. The proportion of clones affected by 1-MCP treatment was much higher in green than in white, pink, and red receptacle tissue. Three major cell-wall-related genes were affected by 1-MCP and, thus, are putatively ethylene dependent: a ripening-repressed beta-galactosidase (Faβgal3), up-regulated by 1-MCP; a putative endo-1,3-1,4-beta-D-glucanase (EGase), up-regulated in green and down-regulated in red fruit by 1-MCP; and a pectate lyase B (plB), expressed only in the red stage and significantly down-regulated by 1-MCP. Furthermore, we have identified genes encoding an alcohol dehydrogenase, a protein kinase-related protein, and a putative glutathione S-transferase, all ripening-induced and down-regulated by 1-MCP, suggesting that their regulation is at least partly ethylene dependent.

Original languageEnglish
Pages (from-to)2088-2090
Number of pages3
JournalHortScience
Volume40
Issue number7
Publication statusPublished - Dec 2005

Fingerprint

1-methylcyclopropene
strawberries
ripening
genes
ethylene
amplified fragment length polymorphism
pectate lyase
ethylene inhibitors
Fragaria
alcohol dehydrogenase
beta-galactosidase
glutathione transferase
protein kinases
fruiting
cell walls
clones
gene expression
fruits
proteins

Keywords

  • cDNA-AFLP
  • Cell wall
  • Fragaria xananassa
  • Gene expression

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

The effect of 1-MCP on the expression of several ripening-related genes in strawberries. / Balogh, Andrea; Koncz, Tímea; Tisza, Viktória; Kiss, E.; Heszky, L.

In: HortScience, Vol. 40, No. 7, 12.2005, p. 2088-2090.

Research output: Contribution to journalArticle

Balogh, Andrea ; Koncz, Tímea ; Tisza, Viktória ; Kiss, E. ; Heszky, L. / The effect of 1-MCP on the expression of several ripening-related genes in strawberries. In: HortScience. 2005 ; Vol. 40, No. 7. pp. 2088-2090.
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