The Drosophila homolog of Aut1 is essential for autophagy and development

G. Juhász, György Csikós, Rita Sinka, M. Erdélyi, M. Sass

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalFEBS Letters
Volume543
Issue number1-3
DOIs
Publication statusPublished - May 22 2003

Fingerprint

Autophagy
Drosophila
Fat Body
RNA Interference
Transgenes
Adipocytes
Yeast
Thermal effects
Larva
Animals
Proteins
Genes
Yeasts
Fats
RNA
Temperature

Keywords

  • Aut1/Apg3
  • Autophagy
  • CG6877/Draut1
  • Drosophila
  • Fat body
  • RNA interference

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The Drosophila homolog of Aut1 is essential for autophagy and development. / Juhász, G.; Csikós, György; Sinka, Rita; Erdélyi, M.; Sass, M.

In: FEBS Letters, Vol. 543, No. 1-3, 22.05.2003, p. 154-158.

Research output: Contribution to journalArticle

Juhász, G. ; Csikós, György ; Sinka, Rita ; Erdélyi, M. ; Sass, M. / The Drosophila homolog of Aut1 is essential for autophagy and development. In: FEBS Letters. 2003 ; Vol. 543, No. 1-3. pp. 154-158.
@article{fd268bccd2084ae595a3a4f08d502825,
title = "The Drosophila homolog of Aut1 is essential for autophagy and development",
abstract = "The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.",
keywords = "Aut1/Apg3, Autophagy, CG6877/Draut1, Drosophila, Fat body, RNA interference",
author = "G. Juh{\'a}sz and Gy{\"o}rgy Csik{\'o}s and Rita Sinka and M. Erd{\'e}lyi and M. Sass",
year = "2003",
month = "5",
day = "22",
doi = "10.1016/S0014-5793(03)00431-9",
language = "English",
volume = "543",
pages = "154--158",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - The Drosophila homolog of Aut1 is essential for autophagy and development

AU - Juhász, G.

AU - Csikós, György

AU - Sinka, Rita

AU - Erdélyi, M.

AU - Sass, M.

PY - 2003/5/22

Y1 - 2003/5/22

N2 - The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.

AB - The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.

KW - Aut1/Apg3

KW - Autophagy

KW - CG6877/Draut1

KW - Drosophila

KW - Fat body

KW - RNA interference

UR - http://www.scopus.com/inward/record.url?scp=0038393057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038393057&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(03)00431-9

DO - 10.1016/S0014-5793(03)00431-9

M3 - Article

C2 - 12753924

AN - SCOPUS:0038393057

VL - 543

SP - 154

EP - 158

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -