The Drosophila homolog of Aut1 is essential for autophagy and development

Gábor Juhász, György Csikós, Rita Sinka, Miklós Erdélyi, Miklós Sass

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The Drosophila homolog of yeast Aut1, CG6877/Draut1, is a ubiquitously expressed cytosolic protein. Draut1 loss of function was achieved by expression of an inverted repeat transgene inducing RNA interference. The effect is temperature-dependent and resembles an allelic series as described by Fortier, E. and Belote, J.M. (Genesis 26 (2000) 240-244). Draut1 loss of function larvae are unable to induce autophagy and heterophagy in fat body cells before pupariation and die during metamorphosis. To our knowledge, this is the first report of a multicellular animal lacking the function of a gene participating in the protein conjugation systems of autophagy.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalFEBS letters
Volume543
Issue number1-3
DOIs
Publication statusPublished - May 22 2003

    Fingerprint

Keywords

  • Aut1/Apg3
  • Autophagy
  • CG6877/Draut1
  • Drosophila
  • Fat body
  • RNA interference

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this