The “disturbing zone” in overpressure layer chromatography (OPLC)

Sz Nyiredy, S. Y. Mészáros, K. Dallenbach‐Toelke, K. Nyiredy‐Mikita, O. Sticher

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27 Citations (Scopus)

Abstract

This paper describes the fundamental factors causing the “disturbing zone” in OPLC. This phenomenon of distorted substance zones appears when the OPLC separation is started with a dry layer and is due to interaction between the gas that is physically bound to the surface of the sorbent (ma) and the gas molecules dissolved in the eluent (ms). Possibilities for elimination of the adverse effects of disturbing zones ultimately depend on the establishment of operating conditions that inhibit their initial formation. Since modification of the location of the disturbing zone is only possible within a very narrow range, the sole solution to this problem is to conduct a pre‐run, which may be carried out with hexane in the case of separation of apolar compounds. For separation of polar substances, the pre‐run may also be performed with hexane or with a solvent of the mobile phase in which the components are unable to migrate. The selection of this solvent may be considered during the optimization of the eluent system.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalJournal of High Resolution Chromatography
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 1987

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Keywords

  • Disturbing zone
  • Overpressure layer chromatography, OPLC
  • “PRISMA” model

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Nyiredy, S., Mészáros, S. Y., Dallenbach‐Toelke, K., Nyiredy‐Mikita, K., & Sticher, O. (1987). The “disturbing zone” in overpressure layer chromatography (OPLC). Journal of High Resolution Chromatography, 10(6), 352-356. https://doi.org/10.1002/jhrc.1240100607