The distribution and aggregatability of intramembrane particles in phenylhydrazine-treated human erythrocytes

Gábor Lelkes, Ildikó Fodor, György Lelkes, S. Hollán, Arie J. Verkleij

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Freeze-fracture analysis of phenylhydrazine-treated, unfixed human erythrocytes showed a random distribution of intramembrane particles both over membrane-bound Heinz-bodies and in the intervening areas when examined after fast freezing in liquid propane. The same results was obtained when unfixed, glycerinated red cells were frozen in liquid Freon. In contrast to previously published data (Low et al. (1985) Science 227, 531-533) these results indicate that binding of Heinz-bodies to the red cell membrane cannot cause morphologically detectable clustering of Band 3 in phenylhydrazine-treated red cells. Over numerous Heinz-bodies a decreased Acridine orange-induced particle aggregation was observed. The phenomenon of the oxidant-induced red cell fluorescence is described.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalBBA - Biomembranes
Volume945
Issue number1
DOIs
Publication statusPublished - Nov 3 1988

Fingerprint

Heinz Bodies
Erythrocytes
Cells
Chlorofluorocarbons
Propane
Acridine Orange
Liquids
Cell membranes
Oxidants
Freezing
Cluster Analysis
Agglomeration
Fluorescence
Cell Membrane
Membranes
phenylhydrazine

Keywords

  • (Human erythrocyte)
  • Fluorescence, oxidant-induced
  • Freeze-fracture
  • Intramembrane particle
  • Phenylhydrazine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

The distribution and aggregatability of intramembrane particles in phenylhydrazine-treated human erythrocytes. / Lelkes, Gábor; Fodor, Ildikó; Lelkes, György; Hollán, S.; Verkleij, Arie J.

In: BBA - Biomembranes, Vol. 945, No. 1, 03.11.1988, p. 105-110.

Research output: Contribution to journalArticle

Lelkes, Gábor ; Fodor, Ildikó ; Lelkes, György ; Hollán, S. ; Verkleij, Arie J. / The distribution and aggregatability of intramembrane particles in phenylhydrazine-treated human erythrocytes. In: BBA - Biomembranes. 1988 ; Vol. 945, No. 1. pp. 105-110.
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