The dipole potential correlates with lipid raft markers in the plasma membrane of living cells

T. Kovács, G. Batta, Florina Zákány, J. Szöllősi, Peter Nagy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The dipole potential generating an electric field much stronger than any other type of membrane potential influences a wide array of phenomena, ranging from passive permeation to voltage-dependent conformational changes of membrane proteins. It is generated by the ordered orientation of lipid carbonyl and membrane-attached water dipole moments. Theoretical considerations and indirect experimental evidence obtained in model membranes suggest that the dipole potential is larger in liquid-ordered domains believed to correspond to lipid rafts in cell membranes. Using three different dipole potential-sensitive fluorophores and four different labeling approaches of raft and nonraft domains, we showed that the dipole potential is indeed stronger in lipid rafts than in the rest of the membrane. The magnitude of this difference is similar to that observed between the dipole potential in control and sphingolipid-enriched cells characteristic of Gaucher’s disease. The results established that the heterogeneity of the dipole potential in living cell membranes is correlated with lipid rafts and imply that alterations in the lipid composition of the cell membrane in human diseases can lead to substantial changes in the dipole potential.

Original languageEnglish
Pages (from-to)1681-1691
Number of pages11
JournalJournal of Lipid Research
Volume58
Issue number8
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Cell membranes
Membrane Lipids
Cells
Cell Membrane
Membrane Microdomains
Lipids
Gaucher Disease
Sphingolipids
Membranes
Membrane Potentials
Membrane Proteins
Water
Fluorophores
Dipole moment
Permeation
Labeling
Electric fields
Liquids
Electric potential
Chemical analysis

Keywords

  • Fluorescence and confocal imaging
  • Fluorescence microscopy
  • Gaucher’s disease
  • Lipid rafts
  • Membrane dipole potential
  • Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

The dipole potential correlates with lipid raft markers in the plasma membrane of living cells. / Kovács, T.; Batta, G.; Zákány, Florina; Szöllősi, J.; Nagy, Peter.

In: Journal of Lipid Research, Vol. 58, No. 8, 01.01.2017, p. 1681-1691.

Research output: Contribution to journalArticle

@article{7cab19e2309c479cb4bd3828384a3bb6,
title = "The dipole potential correlates with lipid raft markers in the plasma membrane of living cells",
abstract = "The dipole potential generating an electric field much stronger than any other type of membrane potential influences a wide array of phenomena, ranging from passive permeation to voltage-dependent conformational changes of membrane proteins. It is generated by the ordered orientation of lipid carbonyl and membrane-attached water dipole moments. Theoretical considerations and indirect experimental evidence obtained in model membranes suggest that the dipole potential is larger in liquid-ordered domains believed to correspond to lipid rafts in cell membranes. Using three different dipole potential-sensitive fluorophores and four different labeling approaches of raft and nonraft domains, we showed that the dipole potential is indeed stronger in lipid rafts than in the rest of the membrane. The magnitude of this difference is similar to that observed between the dipole potential in control and sphingolipid-enriched cells characteristic of Gaucher’s disease. The results established that the heterogeneity of the dipole potential in living cell membranes is correlated with lipid rafts and imply that alterations in the lipid composition of the cell membrane in human diseases can lead to substantial changes in the dipole potential.",
keywords = "Fluorescence and confocal imaging, Fluorescence microscopy, Gaucher’s disease, Lipid rafts, Membrane dipole potential, Membranes",
author = "T. Kov{\'a}cs and G. Batta and Florina Z{\'a}k{\'a}ny and J. Sz{\"o}llősi and Peter Nagy",
year = "2017",
month = "1",
day = "1",
doi = "10.1194/jlr.M077339",
language = "English",
volume = "58",
pages = "1681--1691",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "8",

}

TY - JOUR

T1 - The dipole potential correlates with lipid raft markers in the plasma membrane of living cells

AU - Kovács, T.

AU - Batta, G.

AU - Zákány, Florina

AU - Szöllősi, J.

AU - Nagy, Peter

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The dipole potential generating an electric field much stronger than any other type of membrane potential influences a wide array of phenomena, ranging from passive permeation to voltage-dependent conformational changes of membrane proteins. It is generated by the ordered orientation of lipid carbonyl and membrane-attached water dipole moments. Theoretical considerations and indirect experimental evidence obtained in model membranes suggest that the dipole potential is larger in liquid-ordered domains believed to correspond to lipid rafts in cell membranes. Using three different dipole potential-sensitive fluorophores and four different labeling approaches of raft and nonraft domains, we showed that the dipole potential is indeed stronger in lipid rafts than in the rest of the membrane. The magnitude of this difference is similar to that observed between the dipole potential in control and sphingolipid-enriched cells characteristic of Gaucher’s disease. The results established that the heterogeneity of the dipole potential in living cell membranes is correlated with lipid rafts and imply that alterations in the lipid composition of the cell membrane in human diseases can lead to substantial changes in the dipole potential.

AB - The dipole potential generating an electric field much stronger than any other type of membrane potential influences a wide array of phenomena, ranging from passive permeation to voltage-dependent conformational changes of membrane proteins. It is generated by the ordered orientation of lipid carbonyl and membrane-attached water dipole moments. Theoretical considerations and indirect experimental evidence obtained in model membranes suggest that the dipole potential is larger in liquid-ordered domains believed to correspond to lipid rafts in cell membranes. Using three different dipole potential-sensitive fluorophores and four different labeling approaches of raft and nonraft domains, we showed that the dipole potential is indeed stronger in lipid rafts than in the rest of the membrane. The magnitude of this difference is similar to that observed between the dipole potential in control and sphingolipid-enriched cells characteristic of Gaucher’s disease. The results established that the heterogeneity of the dipole potential in living cell membranes is correlated with lipid rafts and imply that alterations in the lipid composition of the cell membrane in human diseases can lead to substantial changes in the dipole potential.

KW - Fluorescence and confocal imaging

KW - Fluorescence microscopy

KW - Gaucher’s disease

KW - Lipid rafts

KW - Membrane dipole potential

KW - Membranes

UR - http://www.scopus.com/inward/record.url?scp=85026727832&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026727832&partnerID=8YFLogxK

U2 - 10.1194/jlr.M077339

DO - 10.1194/jlr.M077339

M3 - Article

C2 - 28607008

AN - SCOPUS:85026727832

VL - 58

SP - 1681

EP - 1691

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 8

ER -