The development of asthma in children infected with Chlamydia pneumoniae is dependent on the modifying effect of mannose-binding lectin

Adrienne Nagy, G. Kozma, Márton Keszei, András Treszl, A. Falus, C. Szalai

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Background: Although several studies found associations between infection with Chlamydia pneumoniae and asthma, these were mainly restricted to the exacerbation of the symptoms in adults with known asthma. Data about the role of C pneumoniae in the initiation and development of asthma in children are controversial. Objective: We investigated the role of C pneumoniae infection in 139 children with asthma, comparing them with 174 healthy control subjects. Furthermore, we studied the modifying effect of mannose-binding lectin (MBL) variant alleles on the susceptibility to asthma in children infected with C pneumoniae. Methods: C pneumoniae-specific antibodies were measured by means of ELISA, and MBL genotypes were determined by means of PCR-RFLP. Results: There were no significant differences in the percentage of children with positive results for C pneumoniae-specific antibodies between patients and control subjects. Among asthmatic children carrying variant MBL alleles, there were significantly more patients with positive results for C pneumoniae-specific IgG than among control children with variant MBL genotypes (63.7% vs 40.7% of asthmatic vs control children, respectively; odds ratio adjusted for age and sex, 2.21; 95% CI, 1.10-4.41; P = .02). Infected children with variant MBL alleles were found to have a higher risk of asthma development than infected children with normal MBL genotype. This risk was especially high in children with chronic or recurrent infection (positive results for both IgA and IgG; adjusted odds ratio, 5.38; 95% CI, 1.75-14.36; P = .01), but no increased risk was seen in children with current C pneumoniae infection (positive results for IgM). Conclusion: This study indicates the important role of variant MBL alleles in the susceptibility to asthma in children infected with C pneumoniae.

Original languageEnglish
Pages (from-to)729-734
Number of pages6
JournalJournal of Allergy and Clinical Immunology
Volume112
Issue number4
DOIs
Publication statusPublished - Oct 1 2003

Fingerprint

Mannose-Binding Lectin
Chlamydophila pneumoniae
Asthma
Pneumonia
Alleles
Genotype
Infection
Immunoglobulin G
Odds Ratio
Antibodies
Child Development
Restriction Fragment Length Polymorphisms
Immunoglobulin A
Immunoglobulin M
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay

Keywords

  • Bacterial infection
  • Childhood asthma
  • Chlamydia pneumoniae
  • Mannose-binding lectin
  • Polymorphism

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

The development of asthma in children infected with Chlamydia pneumoniae is dependent on the modifying effect of mannose-binding lectin. / Nagy, Adrienne; Kozma, G.; Keszei, Márton; Treszl, András; Falus, A.; Szalai, C.

In: Journal of Allergy and Clinical Immunology, Vol. 112, No. 4, 01.10.2003, p. 729-734.

Research output: Contribution to journalArticle

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abstract = "Background: Although several studies found associations between infection with Chlamydia pneumoniae and asthma, these were mainly restricted to the exacerbation of the symptoms in adults with known asthma. Data about the role of C pneumoniae in the initiation and development of asthma in children are controversial. Objective: We investigated the role of C pneumoniae infection in 139 children with asthma, comparing them with 174 healthy control subjects. Furthermore, we studied the modifying effect of mannose-binding lectin (MBL) variant alleles on the susceptibility to asthma in children infected with C pneumoniae. Methods: C pneumoniae-specific antibodies were measured by means of ELISA, and MBL genotypes were determined by means of PCR-RFLP. Results: There were no significant differences in the percentage of children with positive results for C pneumoniae-specific antibodies between patients and control subjects. Among asthmatic children carrying variant MBL alleles, there were significantly more patients with positive results for C pneumoniae-specific IgG than among control children with variant MBL genotypes (63.7{\%} vs 40.7{\%} of asthmatic vs control children, respectively; odds ratio adjusted for age and sex, 2.21; 95{\%} CI, 1.10-4.41; P = .02). Infected children with variant MBL alleles were found to have a higher risk of asthma development than infected children with normal MBL genotype. This risk was especially high in children with chronic or recurrent infection (positive results for both IgA and IgG; adjusted odds ratio, 5.38; 95{\%} CI, 1.75-14.36; P = .01), but no increased risk was seen in children with current C pneumoniae infection (positive results for IgM). Conclusion: This study indicates the important role of variant MBL alleles in the susceptibility to asthma in children infected with C pneumoniae.",
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AU - Falus, A.

AU - Szalai, C.

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N2 - Background: Although several studies found associations between infection with Chlamydia pneumoniae and asthma, these were mainly restricted to the exacerbation of the symptoms in adults with known asthma. Data about the role of C pneumoniae in the initiation and development of asthma in children are controversial. Objective: We investigated the role of C pneumoniae infection in 139 children with asthma, comparing them with 174 healthy control subjects. Furthermore, we studied the modifying effect of mannose-binding lectin (MBL) variant alleles on the susceptibility to asthma in children infected with C pneumoniae. Methods: C pneumoniae-specific antibodies were measured by means of ELISA, and MBL genotypes were determined by means of PCR-RFLP. Results: There were no significant differences in the percentage of children with positive results for C pneumoniae-specific antibodies between patients and control subjects. Among asthmatic children carrying variant MBL alleles, there were significantly more patients with positive results for C pneumoniae-specific IgG than among control children with variant MBL genotypes (63.7% vs 40.7% of asthmatic vs control children, respectively; odds ratio adjusted for age and sex, 2.21; 95% CI, 1.10-4.41; P = .02). Infected children with variant MBL alleles were found to have a higher risk of asthma development than infected children with normal MBL genotype. This risk was especially high in children with chronic or recurrent infection (positive results for both IgA and IgG; adjusted odds ratio, 5.38; 95% CI, 1.75-14.36; P = .01), but no increased risk was seen in children with current C pneumoniae infection (positive results for IgM). Conclusion: This study indicates the important role of variant MBL alleles in the susceptibility to asthma in children infected with C pneumoniae.

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