The determination of the morphology of melanocytes by laser-generated periodic surface structures

R. Kemkemer, M. Csete, S. Schrank, D. Kaufmann, J. Spatz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We generated self-organized, grating-like structures on the surface of polyethylene-terephthalate by ArF excimer laser ablation. Tilting the polymer films with angles larger than 60°, we got parallel grooves. The surface changes caused by the laser illumination were qualified by AFM. The period of the structures was approximately 1 μm, which is commensurable with the characteristic size of melanocytes. We examined the cell shape of normal epidermal melanocytes (M-C) and of melanocytes from the skin of one neurofibromatosis 1 patient (MNFS) in vitro. The number of dendrites per cell, the length of dendrites and the orientation of several hundred cells were evaluated by an image-processing program. It was proven that the cell morphology and orientation is determined by the topography of the structured polymer substrate. All cells are aligned parallel to the grooves and show the typical bipolar shape. In contrast, on the untreated part of the substrate, the cells are randomly oriented, and the NF cells have more dendrites.

Original languageEnglish
Pages (from-to)437-440
Number of pages4
JournalMaterials Science and Engineering C
Volume23
Issue number3
DOIs
Publication statusPublished - Mar 3 2003

Fingerprint

Surface structure
Polyethylene Terephthalates
Lasers
Excimer lasers
Laser ablation
Substrates
cells
Polymer films
Polyethylene terephthalates
Topography
dendrites
lasers
Skin
Polymers
Image processing
Lighting
grooves
polyethylene terephthalate
polymers
excimer lasers

Keywords

  • Grating
  • Laser-induced structures
  • Melanocytes
  • Morphology
  • Orientation

ASJC Scopus subject areas

  • Biomaterials

Cite this

The determination of the morphology of melanocytes by laser-generated periodic surface structures. / Kemkemer, R.; Csete, M.; Schrank, S.; Kaufmann, D.; Spatz, J.

In: Materials Science and Engineering C, Vol. 23, No. 3, 03.03.2003, p. 437-440.

Research output: Contribution to journalArticle

Kemkemer, R. ; Csete, M. ; Schrank, S. ; Kaufmann, D. ; Spatz, J. / The determination of the morphology of melanocytes by laser-generated periodic surface structures. In: Materials Science and Engineering C. 2003 ; Vol. 23, No. 3. pp. 437-440.
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