The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus

András Táncsics, Tibor Benedek, Sándor Szoboszlay, Péter G. Veres, Milán Farkas, István Máthé, Károly Márialigeti, József Kukolya, Szabolcs Lányi, Balázs Kriszt

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11 Citations (Scopus)

Abstract

Naturally occurring and anthropogenic petroleum hydrocarbons are potential carbon sources for many bacteria. The AlkB-related alkane hydroxylases, which are integral membrane non-heme iron enzymes, play a key role in the microbial degradation of many of these hydrocarbons. Several members of the genus Rhodococcus are well-known alkane degraders and are known to harbor multiple alkB genes encoding for different alkane 1-monooxygenases. In the present study, 48 Rhodococcus strains, representing 35 species of the genus, were investigated to find out whether there was a dominant type of alkB gene widespread among species of the genus that could be used as a phylogenetic marker. Phylogenetic analysis of rhodococcal alkB gene sequences indicated that a certain type of alkB gene was present in almost every member of the genus Rhodococcus. These alkB genes were common in a unique nucleotide sequence stretch absent from other types of rhodococcal alkB genes that encoded a conserved amino acid motif: WLG(I/V/L)D(G/D)GL. The sequence identity of the targeted alkB gene in Rhodococcus ranged from 78.5 to 99.2% and showed higher nucleotide sequence variation at the inter-species level compared to the 16S rRNA gene (93.9-99.8%). The results indicated that the alkB gene type investigated might be applicable for: (i) differentiating closely related Rhodococcus species, (ii) properly assigning environmental isolates to existing Rhodococcus species, and finally (iii) assessing whether a new Rhodococcus isolate represents a novel species of the genus.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalSystematic and Applied Microbiology
Volume38
Issue number1
DOIs
Publication statusPublished - Feb 1 2015

Keywords

  • Alkane 1-monooxygenase
  • Phylogenetic marker gene
  • Rhodococcus

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

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    Táncsics, A., Benedek, T., Szoboszlay, S., Veres, P. G., Farkas, M., Máthé, I., Márialigeti, K., Kukolya, J., Lányi, S., & Kriszt, B. (2015). The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus. Systematic and Applied Microbiology, 38(1), 1-7. https://doi.org/10.1016/j.syapm.2014.10.010