The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus

András Táncsics, Tibor Benedek, Sándor Szoboszlay, Péter G. Veres, Milán Farkas, István Máthé, K. Márialigeti, József Kukolya, Szabolcs Lányi, Balázs Kriszt

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Naturally occurring and anthropogenic petroleum hydrocarbons are potential carbon sources for many bacteria. The AlkB-related alkane hydroxylases, which are integral membrane non-heme iron enzymes, play a key role in the microbial degradation of many of these hydrocarbons. Several members of the genus Rhodococcus are well-known alkane degraders and are known to harbor multiple alkB genes encoding for different alkane 1-monooxygenases. In the present study, 48 Rhodococcus strains, representing 35 species of the genus, were investigated to find out whether there was a dominant type of alkB gene widespread among species of the genus that could be used as a phylogenetic marker. Phylogenetic analysis of rhodococcal alkB gene sequences indicated that a certain type of alkB gene was present in almost every member of the genus Rhodococcus. These alkB genes were common in a unique nucleotide sequence stretch absent from other types of rhodococcal alkB genes that encoded a conserved amino acid motif: WLG(I/V/L)D(G/D)GL. The sequence identity of the targeted alkB gene in Rhodococcus ranged from 78.5 to 99.2% and showed higher nucleotide sequence variation at the inter-species level compared to the 16S rRNA gene (93.9-99.8%). The results indicated that the alkB gene type investigated might be applicable for: (i) differentiating closely related Rhodococcus species, (ii) properly assigning environmental isolates to existing Rhodococcus species, and finally (iii) assessing whether a new Rhodococcus isolate represents a novel species of the genus.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalSystematic and Applied Microbiology
Volume38
Issue number1
DOIs
Publication statusPublished - Feb 1 2015

Fingerprint

Cytochrome P-450 CYP4A
Rhodococcus (bacteria)
Rhodococcus
alkane
alkanes
phylogenetics
gene
phylogeny
Genes
genes
Hydrocarbons
nucleotide sequences
hydrocarbons
Dominant Genes
Amino Acid Motifs
amino acid motifs
Alkanes
Petroleum
detection
analysis

Keywords

  • Alkane 1-monooxygenase
  • Phylogenetic marker gene.
  • Rhodococcus

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus. / Táncsics, András; Benedek, Tibor; Szoboszlay, Sándor; Veres, Péter G.; Farkas, Milán; Máthé, István; Márialigeti, K.; Kukolya, József; Lányi, Szabolcs; Kriszt, Balázs.

In: Systematic and Applied Microbiology, Vol. 38, No. 1, 01.02.2015, p. 1-7.

Research output: Contribution to journalArticle

Táncsics, A, Benedek, T, Szoboszlay, S, Veres, PG, Farkas, M, Máthé, I, Márialigeti, K, Kukolya, J, Lányi, S & Kriszt, B 2015, 'The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus', Systematic and Applied Microbiology, vol. 38, no. 1, pp. 1-7. https://doi.org/10.1016/j.syapm.2014.10.010
Táncsics, András ; Benedek, Tibor ; Szoboszlay, Sándor ; Veres, Péter G. ; Farkas, Milán ; Máthé, István ; Márialigeti, K. ; Kukolya, József ; Lányi, Szabolcs ; Kriszt, Balázs. / The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus. In: Systematic and Applied Microbiology. 2015 ; Vol. 38, No. 1. pp. 1-7.
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