The detailed physical map of the temperate phage 16-3 of Rhizobium meliloti 41

László Dorgai, Gábor Polner, Erzsébet Jónás, Nándor Garamszegi, Zoltán Ascher, Anikó Páy, Géza Dallmann, László Orosz

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Abstract

Restriction cleavage maps for enzymes EcoRI, BamHI, PstI, PvuII, XbaI and EcoRV of Rhizobium meliloti temparate phage 16-3 have been established. Together with the earlier maps (HindIII, KpnI, HpaI, BglII) 98 restriction sites, 'evenly' distributed, have been mapped along the phage genome, including the so far unmarked silent region of the chromosome. All the restriction maps have been fitted to each other by computer optimalization. Beyond the conventional techniques a computer program (PMAP) for physical mapping of linear DNA has been employed which made the experimentation, in several cases, extremely efficient.

Original languageEnglish
Pages (from-to)430-433
Number of pages4
JournalMGG Molecular & General Genetics
Volume191
Issue number3
DOIs
Publication statusPublished - Jan 1 1983

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ASJC Scopus subject areas

  • Genetics

Cite this

Dorgai, L., Polner, G., Jónás, E., Garamszegi, N., Ascher, Z., Páy, A., Dallmann, G., & Orosz, L. (1983). The detailed physical map of the temperate phage 16-3 of Rhizobium meliloti 41. MGG Molecular & General Genetics, 191(3), 430-433. https://doi.org/10.1007/BF00425759