The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp.

Andrew Plumridge, Petter Melin, Malcolm Stratford, Michaela Novodvorska, Lee Shunburne, Paul S. Dyer, J. Roubos, Hildegard Menke, Jacques Stark, Hein Stam, David B. Archer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadienoic acid) to 1,3-pentadiene. It is now shown that this decarboxylation process also requires the activity of a putative 4-hydroxybenzoic acid (3-octaprenyl-4-hydroxybenzoic acid) decarboxylase, encoded by a gene termed ohbA1, and a putative transcription factor, sorbic acid decarboxylase regulator, encoded by sdrA. The padA1, ohbA1 and sdrA genes are in close proximity to each other on chromosome 6 in the A. niger genome and further bioinformatic analysis revealed conserved synteny at this locus in several Aspergillus species and other ascomycete fungi indicating clustering of metabolic function. This cluster is absent from the genomes of A. fumigatus and A. clavatus and, as a consequence, neither species is capable of decarboxylating sorbic acid.

Original languageEnglish
Pages (from-to)683-692
Number of pages10
JournalFungal Genetics and Biology
Volume47
Issue number8
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Sorbic Acid
Decarboxylation
Aspergillus
Acids
Carboxy-Lyases
Aspergillus niger
Genes
Genome
Synteny
Ascomycota
Chromosomes, Human, Pair 6
Computational Biology
Cluster Analysis
Fungi
Transcription Factors
4-hydroxybenzoic acid

Keywords

  • 1,3-Pentadiene
  • Aspergillus
  • Cinnamic acid
  • Conidia
  • Decarboxylation
  • Gene regulation
  • Germination
  • Saccharomyces
  • Sorbic acid
  • Styrene

ASJC Scopus subject areas

  • Genetics
  • Microbiology
  • Medicine(all)

Cite this

Plumridge, A., Melin, P., Stratford, M., Novodvorska, M., Shunburne, L., Dyer, P. S., ... Archer, D. B. (2010). The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp. Fungal Genetics and Biology, 47(8), 683-692. https://doi.org/10.1016/j.fgb.2010.04.011

The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp. / Plumridge, Andrew; Melin, Petter; Stratford, Malcolm; Novodvorska, Michaela; Shunburne, Lee; Dyer, Paul S.; Roubos, J.; Menke, Hildegard; Stark, Jacques; Stam, Hein; Archer, David B.

In: Fungal Genetics and Biology, Vol. 47, No. 8, 08.2010, p. 683-692.

Research output: Contribution to journalArticle

Plumridge, A, Melin, P, Stratford, M, Novodvorska, M, Shunburne, L, Dyer, PS, Roubos, J, Menke, H, Stark, J, Stam, H & Archer, DB 2010, 'The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp.', Fungal Genetics and Biology, vol. 47, no. 8, pp. 683-692. https://doi.org/10.1016/j.fgb.2010.04.011
Plumridge, Andrew ; Melin, Petter ; Stratford, Malcolm ; Novodvorska, Michaela ; Shunburne, Lee ; Dyer, Paul S. ; Roubos, J. ; Menke, Hildegard ; Stark, Jacques ; Stam, Hein ; Archer, David B. / The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp. In: Fungal Genetics and Biology. 2010 ; Vol. 47, No. 8. pp. 683-692.
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