The current status of species recognition and identification in Aspergillus

D. M. Geiser, M. A. Klich, J. C. Frisvad, S. W. Peterson, J. Varga, R. A. Samson

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

The species recognition and identification of aspergilli and their teleomorphs is discussed. A historical overview of the taxonomic concepts starting with the monograph of Raper & Fennell (1965) is given. A list of taxa described since 2000 is provided. Physiological characters, particularly growth rates and the production of extrolites, often show differences that reflect phylogenetic species boundaries and greater emphasis should be placed on extrolite profiles and growth characteristics in species descriptions. Multilocus sequence-based phylogenetic analyses have emerged as the primary tool for inferring phylogenetic species boundaries and relationships within subgenera and sections. A four locus DNA sequence study covering all major lineages in Aspergillus using genealogical concordance theory resulted in a species recognition system that agrees in part with phenotypic studies and reveals the presence of many undescribed species not resolved by phenotype. The use of as much data from as many sources as possible in making taxonomic decisions is advocated. For species identification, DNA barcoding uses a short genetic marker in an organism's DNA to quickly and easily identify it to a particular species. Partial cytochrome oxidase subunit 1 sequences, which are used for barcoding animal species, were found to have limited value for species identification among black aspergilli. The various possibilities are discussed and at present partial β-tubulin or calmodulin are the most promising loci for Aspergillus identification. For characterising Aspergillus species one application would be to produce a multilocus phylogeny, with the goal of having a firm understanding of the evolutionary relationships among species across the entire genus. DNA chip technologies are discussed as possibilities for an accurate multilocus barcoding tool for the genus Aspergillus.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalStudies in Mycology
Volume59
DOIs
Publication statusPublished - 2007

Fingerprint

Aspergillus
barcoding
phylogeny
teleomorphs
loci
DNA
DNA barcoding
calmodulin
Electron Transport Complex IV
Calmodulin
Phylogeny
Tubulin
Growth
tubulin
Oligonucleotide Array Sequence Analysis
Genetic Markers
cytochrome-c oxidase
Recognition (Psychology)
Identification (Psychology)
Decision Making

Keywords

  • Multolocus phylogenetics
  • Polyphasic taxonomy
  • Species identification
  • Species recognition

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Geiser, D. M., Klich, M. A., Frisvad, J. C., Peterson, S. W., Varga, J., & Samson, R. A. (2007). The current status of species recognition and identification in Aspergillus. Studies in Mycology, 59, 1-10. https://doi.org/10.3114/sim.2007.59.01

The current status of species recognition and identification in Aspergillus. / Geiser, D. M.; Klich, M. A.; Frisvad, J. C.; Peterson, S. W.; Varga, J.; Samson, R. A.

In: Studies in Mycology, Vol. 59, 2007, p. 1-10.

Research output: Contribution to journalArticle

Geiser, D. M. ; Klich, M. A. ; Frisvad, J. C. ; Peterson, S. W. ; Varga, J. ; Samson, R. A. / The current status of species recognition and identification in Aspergillus. In: Studies in Mycology. 2007 ; Vol. 59. pp. 1-10.
@article{692d89066b6e4b08815ddeadef332da9,
title = "The current status of species recognition and identification in Aspergillus",
abstract = "The species recognition and identification of aspergilli and their teleomorphs is discussed. A historical overview of the taxonomic concepts starting with the monograph of Raper & Fennell (1965) is given. A list of taxa described since 2000 is provided. Physiological characters, particularly growth rates and the production of extrolites, often show differences that reflect phylogenetic species boundaries and greater emphasis should be placed on extrolite profiles and growth characteristics in species descriptions. Multilocus sequence-based phylogenetic analyses have emerged as the primary tool for inferring phylogenetic species boundaries and relationships within subgenera and sections. A four locus DNA sequence study covering all major lineages in Aspergillus using genealogical concordance theory resulted in a species recognition system that agrees in part with phenotypic studies and reveals the presence of many undescribed species not resolved by phenotype. The use of as much data from as many sources as possible in making taxonomic decisions is advocated. For species identification, DNA barcoding uses a short genetic marker in an organism's DNA to quickly and easily identify it to a particular species. Partial cytochrome oxidase subunit 1 sequences, which are used for barcoding animal species, were found to have limited value for species identification among black aspergilli. The various possibilities are discussed and at present partial β-tubulin or calmodulin are the most promising loci for Aspergillus identification. For characterising Aspergillus species one application would be to produce a multilocus phylogeny, with the goal of having a firm understanding of the evolutionary relationships among species across the entire genus. DNA chip technologies are discussed as possibilities for an accurate multilocus barcoding tool for the genus Aspergillus.",
keywords = "Multolocus phylogenetics, Polyphasic taxonomy, Species identification, Species recognition",
author = "Geiser, {D. M.} and Klich, {M. A.} and Frisvad, {J. C.} and Peterson, {S. W.} and J. Varga and Samson, {R. A.}",
year = "2007",
doi = "10.3114/sim.2007.59.01",
language = "English",
volume = "59",
pages = "1--10",
journal = "Studies in Mycology",
issn = "0166-0616",
publisher = "Centraalbureau voor Schimmelculturen",

}

TY - JOUR

T1 - The current status of species recognition and identification in Aspergillus

AU - Geiser, D. M.

AU - Klich, M. A.

AU - Frisvad, J. C.

AU - Peterson, S. W.

AU - Varga, J.

AU - Samson, R. A.

PY - 2007

Y1 - 2007

N2 - The species recognition and identification of aspergilli and their teleomorphs is discussed. A historical overview of the taxonomic concepts starting with the monograph of Raper & Fennell (1965) is given. A list of taxa described since 2000 is provided. Physiological characters, particularly growth rates and the production of extrolites, often show differences that reflect phylogenetic species boundaries and greater emphasis should be placed on extrolite profiles and growth characteristics in species descriptions. Multilocus sequence-based phylogenetic analyses have emerged as the primary tool for inferring phylogenetic species boundaries and relationships within subgenera and sections. A four locus DNA sequence study covering all major lineages in Aspergillus using genealogical concordance theory resulted in a species recognition system that agrees in part with phenotypic studies and reveals the presence of many undescribed species not resolved by phenotype. The use of as much data from as many sources as possible in making taxonomic decisions is advocated. For species identification, DNA barcoding uses a short genetic marker in an organism's DNA to quickly and easily identify it to a particular species. Partial cytochrome oxidase subunit 1 sequences, which are used for barcoding animal species, were found to have limited value for species identification among black aspergilli. The various possibilities are discussed and at present partial β-tubulin or calmodulin are the most promising loci for Aspergillus identification. For characterising Aspergillus species one application would be to produce a multilocus phylogeny, with the goal of having a firm understanding of the evolutionary relationships among species across the entire genus. DNA chip technologies are discussed as possibilities for an accurate multilocus barcoding tool for the genus Aspergillus.

AB - The species recognition and identification of aspergilli and their teleomorphs is discussed. A historical overview of the taxonomic concepts starting with the monograph of Raper & Fennell (1965) is given. A list of taxa described since 2000 is provided. Physiological characters, particularly growth rates and the production of extrolites, often show differences that reflect phylogenetic species boundaries and greater emphasis should be placed on extrolite profiles and growth characteristics in species descriptions. Multilocus sequence-based phylogenetic analyses have emerged as the primary tool for inferring phylogenetic species boundaries and relationships within subgenera and sections. A four locus DNA sequence study covering all major lineages in Aspergillus using genealogical concordance theory resulted in a species recognition system that agrees in part with phenotypic studies and reveals the presence of many undescribed species not resolved by phenotype. The use of as much data from as many sources as possible in making taxonomic decisions is advocated. For species identification, DNA barcoding uses a short genetic marker in an organism's DNA to quickly and easily identify it to a particular species. Partial cytochrome oxidase subunit 1 sequences, which are used for barcoding animal species, were found to have limited value for species identification among black aspergilli. The various possibilities are discussed and at present partial β-tubulin or calmodulin are the most promising loci for Aspergillus identification. For characterising Aspergillus species one application would be to produce a multilocus phylogeny, with the goal of having a firm understanding of the evolutionary relationships among species across the entire genus. DNA chip technologies are discussed as possibilities for an accurate multilocus barcoding tool for the genus Aspergillus.

KW - Multolocus phylogenetics

KW - Polyphasic taxonomy

KW - Species identification

KW - Species recognition

UR - http://www.scopus.com/inward/record.url?scp=38549114657&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38549114657&partnerID=8YFLogxK

U2 - 10.3114/sim.2007.59.01

DO - 10.3114/sim.2007.59.01

M3 - Article

C2 - 18490947

AN - SCOPUS:38549114657

VL - 59

SP - 1

EP - 10

JO - Studies in Mycology

JF - Studies in Mycology

SN - 0166-0616

ER -