The C2H2 transcription factor REGULATOR OF SYMBIOSOME DIFFERENTIATION represses transcription of the secretory pathway gene VAMP721a and promotes symbiosome development in Medicago truncatula

Senjuti Sinharoy, Ivone Torres-Jerez, Kaustav Bandyopadhyay, A. Kereszt, Catalina I. Pislariu, Jin Nakashima, Vagner A. Benedito, É. Kondorosi, Michael K. Udvardi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Transcription factors (TFs) are thought to regulate many aspects of nodule and symbiosis development in legumes, although few TFs have been characterized functionally. Here, we describe REGULATOR OF SYMBIOSOME DIFFERENTIATION (RSD) of Medicago truncatula, a member of the Cysteine-2/Histidine-2 (C2H2) family of plant TFs that is required for normal symbiosome differentiation during nodule development. RSD is expressed in a nodule-specific manner, with maximal transcript levels in the bacterial invasion zone. A tobacco (Nicotiana tabacum) retrotransposon (Tnt1) insertion rsd mutant produced nodules that were unable to fix nitrogen and that contained incompletely differentiated symbiosomes and bacteroids. RSD protein was localized to the nucleus, consistent with a role of the protein in transcriptional regulation. RSD acted as a transcriptional repressor in a heterologous yeast assay. Transcriptome analysis of an rsd mutant identified 11 genes as potential targets of RSD repression. RSD interacted physically with the promoter of one of these genes, VAMP721a, which encodes vesicle-associated membrane protein 721a. Thus, RSD may influence symbiosome development in part by repressing transcription of VAMP721a and modifying vesicle trafficking in nodule cells. This establishes RSD as a TF implicated directly in symbiosome and bacteroid differentiation and a transcriptional regulator of secretory pathway genes in plants.

Original languageEnglish
Pages (from-to)3584-3601
Number of pages18
JournalPlant Cell
Volume25
Issue number9
DOIs
Publication statusPublished - Sep 2013

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Medicago truncatula
Secretory Pathway
histidine
Histidine
Cysteine
cysteine
Transcription Factors
transcription factors
transcription (genetics)
Genes
Tobacco
genes
R-SNARE Proteins
Plant Genes
Retroelements
Symbiosis
Gene Expression Profiling
mutants
Fabaceae
retrotransposons

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Medicine(all)

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The C2H2 transcription factor REGULATOR OF SYMBIOSOME DIFFERENTIATION represses transcription of the secretory pathway gene VAMP721a and promotes symbiosome development in Medicago truncatula. / Sinharoy, Senjuti; Torres-Jerez, Ivone; Bandyopadhyay, Kaustav; Kereszt, A.; Pislariu, Catalina I.; Nakashima, Jin; Benedito, Vagner A.; Kondorosi, É.; Udvardi, Michael K.

In: Plant Cell, Vol. 25, No. 9, 09.2013, p. 3584-3601.

Research output: Contribution to journalArticle

Sinharoy, Senjuti ; Torres-Jerez, Ivone ; Bandyopadhyay, Kaustav ; Kereszt, A. ; Pislariu, Catalina I. ; Nakashima, Jin ; Benedito, Vagner A. ; Kondorosi, É. ; Udvardi, Michael K. / The C2H2 transcription factor REGULATOR OF SYMBIOSOME DIFFERENTIATION represses transcription of the secretory pathway gene VAMP721a and promotes symbiosome development in Medicago truncatula. In: Plant Cell. 2013 ; Vol. 25, No. 9. pp. 3584-3601.
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