Human erythrocytes were frozen at -20, -40, -80, and -196°C in the presence of glycerol as protecting agent. The biochemical parameters of erythrocytes were studied immediately after thawing and washing. K+ and ATP content, membrane phospholipids and proteins, as well as the morphological and osmotic properties, were determined.
|Number of pages||8|
|Publication status||Published - Dec 1 1972|
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