The comparison of two different extenders for the improvement of large-scale sperm cryopreservation in common carp (Cyprinus carpio)

Levente Várkonyi, Zoltán Bokor, József Molnár, Ferenc Fodor, Zsolt Szári, Árpád Ferincz, Ádám Staszny, Levente Zete Láng, Balázs Csorbai, B. Urbányi, Gergely Bernáth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large-scale (elevated volume of sperm) freezing method in a controlled-rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled-rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

Original languageEnglish
JournalReproduction in Domestic Animals
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Esocidae
pike
Carps
Cryopreservation
Cyprinus carpio
cryopreservation
Spermatozoa
spermatozoa
straw
freezers
Polystyrenes
polystyrenes
agglutination
carp
thawing
Freezing
Sperm Agglutination
freezing
Agglutination
cell suspension culture

Keywords

  • CASA
  • common carp
  • large-scale cryopreservation
  • motility parameters
  • sperm agglutination

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Endocrinology

Cite this

The comparison of two different extenders for the improvement of large-scale sperm cryopreservation in common carp (Cyprinus carpio). / Várkonyi, Levente; Bokor, Zoltán; Molnár, József; Fodor, Ferenc; Szári, Zsolt; Ferincz, Árpád; Staszny, Ádám; Láng, Levente Zete; Csorbai, Balázs; Urbányi, B.; Bernáth, Gergely.

In: Reproduction in Domestic Animals, 01.01.2018.

Research output: Contribution to journalArticle

Várkonyi, Levente ; Bokor, Zoltán ; Molnár, József ; Fodor, Ferenc ; Szári, Zsolt ; Ferincz, Árpád ; Staszny, Ádám ; Láng, Levente Zete ; Csorbai, Balázs ; Urbányi, B. ; Bernáth, Gergely. / The comparison of two different extenders for the improvement of large-scale sperm cryopreservation in common carp (Cyprinus carpio). In: Reproduction in Domestic Animals. 2018.
@article{350e57918e4f45db80901b7190dbb711,
title = "The comparison of two different extenders for the improvement of large-scale sperm cryopreservation in common carp (Cyprinus carpio)",
abstract = "In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large-scale (elevated volume of sperm) freezing method in a controlled-rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54{\%} ± 8{\%}, Pike: 37{\%} ± 5{\%}), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled-rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.",
keywords = "CASA, common carp, large-scale cryopreservation, motility parameters, sperm agglutination",
author = "Levente V{\'a}rkonyi and Zolt{\'a}n Bokor and J{\'o}zsef Moln{\'a}r and Ferenc Fodor and Zsolt Sz{\'a}ri and {\'A}rp{\'a}d Ferincz and {\'A}d{\'a}m Staszny and L{\'a}ng, {Levente Zete} and Bal{\'a}zs Csorbai and B. Urb{\'a}nyi and Gergely Bern{\'a}th",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/rda.13383",
language = "English",
journal = "Reproduction in Domestic Animals",
issn = "0936-6768",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - The comparison of two different extenders for the improvement of large-scale sperm cryopreservation in common carp (Cyprinus carpio)

AU - Várkonyi, Levente

AU - Bokor, Zoltán

AU - Molnár, József

AU - Fodor, Ferenc

AU - Szári, Zsolt

AU - Ferincz, Árpád

AU - Staszny, Ádám

AU - Láng, Levente Zete

AU - Csorbai, Balázs

AU - Urbányi, B.

AU - Bernáth, Gergely

PY - 2018/1/1

Y1 - 2018/1/1

N2 - In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large-scale (elevated volume of sperm) freezing method in a controlled-rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled-rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

AB - In our study, a traditionally used (Grayling, already used in cyprinid species) and a newly tested (Pike) extender was tested to avoid sperm agglutination phenomenon following thawing during carp sperm cryopreservation. A large-scale (elevated volume of sperm) freezing method in a controlled-rate freezer using 5 ml straw and 10 ml cryotube was also systematically established. In all experiments, the sperm cryopreserved in using Grayling extender (except only one sample) showed an agglutination phenomenon (damaged and intact cells adhered to each other) after thawing where Pike extender resulted the regular cell suspension. No significant difference was observed between the two cryopreserved groups (Pike and Grayling extender) in all motility parameters using the 0.5 ml straw and the polystyrene box. Similarly, motility parameters did not show a significant difference in the two frozen groups with the 5 ml straw, also in the polystyrene box. A significantly higher progressive motility (pMOT, Grayling: 54% ± 8%, Pike: 37% ± 5%), straight line velocity (VSL, Grayling: 50 ± 5 µm/s, Pike: 39 ± 4 µm/s) and beat cross frequency (BCF, Grayling: 20 ± 1 Hz, Pike: 17 ± 1 Hz) was observed in the case of the grayling extender by the 5 ml straw cryopreserved in a controlled-rate freezer (CRF) compare to the pike extender. A significantly higher VSL (Grayling: 45 ± 3 µm/s, Pike: 38 ± 4 µm/s) was observed by the grayling extender using the 10 ml cryotube than with the pike extender. Despite the randomly occurring differences in a few parameters, our new controlled freezing method using the newly tested Pike extender, the 5 ml straw or the 10 ml cryotube can be a good solution for the preservation of elevated volume of carp sperm.

KW - CASA

KW - common carp

KW - large-scale cryopreservation

KW - motility parameters

KW - sperm agglutination

UR - http://www.scopus.com/inward/record.url?scp=85058416344&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85058416344&partnerID=8YFLogxK

U2 - 10.1111/rda.13383

DO - 10.1111/rda.13383

M3 - Article

JO - Reproduction in Domestic Animals

JF - Reproduction in Domestic Animals

SN - 0936-6768

ER -