The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation

E. Tyihák, L. Botz, P. Ott, S. Nagy, B. Kocsis, Z. Király-Véghely, E. Mincsovics

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

BioArena is a complex separation and detection system, which integrates the advantages of the overpressured layer chromatography (OPLC) and bioautography. It exploits the interaction possibilities between the microbes and the dye substance as well as other small and big co-factor molecules in the sorbent bed after separation. OPLC separation resulted in the increase of the sensitivity and the compactness of the spots in comparison to the conventional TLC-HPTLC separations. OPLC and bioautography as a detection system facilitate studying different biological interactions in the field of antibiosis, especially model reactions of endogenous formaldehyde (HCHO) and other small endogenous molecules (e.g. H2O2), as well as trans-resveratrol.

Original languageEnglish
Pages (from-to)543-553
Number of pages11
JournalChemia Analityczna
Volume48
Issue number3
Publication statusPublished - 2003

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Cell proliferation
Chromatography
Molecules
Sorbents
Formaldehyde
Coloring Agents

Keywords

  • BioArena
  • Bioautography
  • Cell proliferation
  • OPLC
  • Resveratrol
  • TLC

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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AU - Tyihák, E.

AU - Botz, L.

AU - Ott, P.

AU - Nagy, S.

AU - Kocsis, B.

AU - Király-Véghely, Z.

AU - Mincsovics, E.

PY - 2003

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