The combination of pamidronate and calcitriol reverses particle- and TNF-α-induced altered functions of bone-marrow-derived stromal cells with osteoblastic

C. Vermes, R. Chandrasekaran, J. G. Dobai, J. J. Jacobs, G. B J Andersson, H. An, N. J. Hallab, J. O. Galante, T. T. Glant

Research output: Contribution to journalArticle

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Abstract

Periprosthetic bone loss after total joint arthroplasty is a major clinical problem resulting in aseptic loosening of the implant. Among many cell types, osteoblasts play a crucial role in the development of peri-implant osteolysis. In this study, we tested the effects of calcitriol (1α,25-dihydroxy-vitamin-D3) and the bisphosphonate pamidronate on titanium-particle-and TNF-α-induced release of interleukin-6 and suppression of osteoblast-specific gene expressions in bone-marrow-derived stromal cells with an osteoblastic phenotype. We monitored the expression of procollagen α1[1], osteocalcin, osteonectin and alkaline phosphatase mRNAs by Northern blots and real-time reverse transcription and polymerase chain reaction analyses. The release of various cytokines was also analysed by ELISA. We found that calcitriol or pamidronate could only partially recover the altered functions of osteoblasts when added alone. Only a combination of these compounds restored all the tested functions of osteoblasts. The local delivery of these drugs may have therapeutic potential to prevent or to treat periprosthetic osteolysis and aseptic loosening of implants.

Original languageEnglish
Pages (from-to)759-770
Number of pages12
JournalJournal of Bone and Joint Surgery - Series B
Volume86
Issue number5
DOIs
Publication statusPublished - Jul 2004

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pamidronate
Calcitriol
Osteoblasts
Mesenchymal Stromal Cells
Osteolysis
Osteonectin
Procollagen
Osteocalcin
Diphosphonates
Titanium
Arthroplasty
Northern Blotting
Reverse Transcription
Alkaline Phosphatase
Interleukin-6
Joints
Enzyme-Linked Immunosorbent Assay
Cytokines
Phenotype
Gene Expression

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

The combination of pamidronate and calcitriol reverses particle- and TNF-α-induced altered functions of bone-marrow-derived stromal cells with osteoblastic. / Vermes, C.; Chandrasekaran, R.; Dobai, J. G.; Jacobs, J. J.; Andersson, G. B J; An, H.; Hallab, N. J.; Galante, J. O.; Glant, T. T.

In: Journal of Bone and Joint Surgery - Series B, Vol. 86, No. 5, 07.2004, p. 759-770.

Research output: Contribution to journalArticle

Vermes, C. ; Chandrasekaran, R. ; Dobai, J. G. ; Jacobs, J. J. ; Andersson, G. B J ; An, H. ; Hallab, N. J. ; Galante, J. O. ; Glant, T. T. / The combination of pamidronate and calcitriol reverses particle- and TNF-α-induced altered functions of bone-marrow-derived stromal cells with osteoblastic. In: Journal of Bone and Joint Surgery - Series B. 2004 ; Vol. 86, No. 5. pp. 759-770.
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