The colocalization of parvalbumin and calbindin-D28k with GABA in the subnucleus caudalis of the rat spinal trigeminal nucleus

E. Polgár, M. Antal

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The colocalization of two calcium-binding proteins, parvalbumin (PV) and calbindin-D28k (CaB), which have been reported to be markers of specific subpopulations of neurons in the central nervous system, with the inhibitory amino acid neurotransmitter γ-amino-butyric acid (GABA) was investigated in neurons of laminae I-IV of the subnucleus caudalis of the rat spinal trigeminal nucleus by using post-embedding immunocytochemical methods. Cells immunoreactive for PV, CaB, and GABA were found in all four laminae of the subnucleus caudalis. A substantial proportion of PV-immunoreactive perikarya were also stained for GABA in laminae II and III (44.8% and 39.8%, respectively). However, the majority of PV-containing neurons in laminae I and IV (100% and 86%, respectively), as well as CaB-immunoreactive cells in all four laminae (98.4%), were GABA-negative. These results show that, in contrast to higher brain centers, PV-, CaB-, and GABA-immunoreactive perikarya represent significantly different populations of neurons in the subnucleus caudalis of the rat. In the light of the present findings, the differences in the neurochemical properties of the subnucleus caudalis of the spinal trigeminal nucleus and the spinal dorsal horn are also discussed.

Original languageEnglish
Pages (from-to)402-408
Number of pages7
JournalExperimental Brain Research
Volume103
Issue number3
DOIs
Publication statusPublished - Nov 1995

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Calbindin 1
Spinal Trigeminal Nucleus
Parvalbumins
gamma-Aminobutyric Acid
Neurons
Substantia Gelatinosa
Calcium-Binding Proteins
Butyric Acid
Neurotransmitter Agents
Central Nervous System
Spinal Cord Dorsal Horn
Amino Acids
Brain
Population

Keywords

  • Calcium-binding proteins
  • GABA Immunocytochemistry
  • Medullary dorsal horn
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The colocalization of parvalbumin and calbindin-D28k with GABA in the subnucleus caudalis of the rat spinal trigeminal nucleus. / Polgár, E.; Antal, M.

In: Experimental Brain Research, Vol. 103, No. 3, 11.1995, p. 402-408.

Research output: Contribution to journalArticle

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