The chromophore structure and chromophore-protein interactions in C-phycocyanin as studied by resonance Raman spectroscopy

B. Szalontai, Z. Gombos, Vilmos Csizmadia, Marc Lutz

Research output: Contribution to journalArticle

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Abstract

We have studied the changes occurring in the electronic absorption and resonance Raman spectra of C-phycocyanin from Synechococcus 6301 (Anacystis nidulans) during denaturation of the protein induced by lowering the pH of the medium. Absorption studies showed that phycocyanobilin probably does not change its native, extended conformation in the pH range 7.5-3.0, but folds into a helical conformation as the pH is lowered further. Resonance Raman spectra obtained using ultraviolet excitation at 363.8 nm and at low temperature (40 K) revealed that non-covalent chromophore-protein interactions which are necessary in maintaining the extended conformation of the phycocyanobilin chromophore in the native protein involve the ends of the chromophore. They probably involve strong interactions from the protein on the two lactam C=O groups. These groups indeed become protonated only at pH values lower than 2.7, hence allowing folding of the chromophore. At pH 1.5 a complete denaturation of the protein occurs, but even this is partly reversible upon restoration of the physiological pH values.

Original languageEnglish
Pages (from-to)296-304
Number of pages9
JournalBBA - Bioenergetics
Volume893
Issue number2
DOIs
Publication statusPublished - Sep 10 1987

Fingerprint

Phycocyanin
Raman Spectrum Analysis
Chromophores
Raman spectroscopy
Conformations
Denaturation
Protein Denaturation
Proteins
Raman scattering
Lactams
Synechococcus
Restoration
Temperature

Keywords

  • (A. nidulans)
  • Absorption spectroscopy
  • Antenna complex
  • Photosynthesis
  • Phycobilisome
  • Resonance Raman spectroscopy

ASJC Scopus subject areas

  • Biophysics

Cite this

The chromophore structure and chromophore-protein interactions in C-phycocyanin as studied by resonance Raman spectroscopy. / Szalontai, B.; Gombos, Z.; Csizmadia, Vilmos; Lutz, Marc.

In: BBA - Bioenergetics, Vol. 893, No. 2, 10.09.1987, p. 296-304.

Research output: Contribution to journalArticle

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