The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction

A. Pfeiffer, M. Kapinsky, E. Orsó, G. Schütz, H. Schindler, P. Nagy, G. Rothe, J. Szöllősi, S. Damjanovich, G. Schmitz

Research output: Contribution to journalArticle

Abstract

Lipopolysaccharide (LPS) is known to bind to several surface molecules on various cells. The best characterized LPS-binding protein is CD14, strongly expressed in the majority of monocytes. Since CD14 is a glycosylphosphatidyl-inositol (GPI)-anchor protein without a cytoplasmic tail, it has been suggested that additional signalling receptors co-associate with CD14 in order to initiate signal transduction cascades. Here we show that after ligand binding in human blood monocytes, the b2-integrin CD11b/CD18 forms clusters with CD14, after ligand binding, Thus, in response to LPS stimulation a receptor complex is formed in the plane of the plasma membrane which may be of central importance for cellular responses to LPS. We also show that another physiologically relevant ligand of CD14, ceramide, which is structurally similar to LPS, induces a complex of CD14 and CD11b/CD18 after binding to CD14.

Original languageEnglish
Pages (from-to)113-115
Number of pages3
JournalSingle Molecules
Volume2
Issue number2
Publication statusPublished - 2001

Fingerprint

Signal transduction
Cell membranes
Lipopolysaccharides
Signal Transduction
Cholesterol
Cell Membrane
Ligands
Monocytes
Ceramides
Inositol
Anchors
Integrins
Tail
Blood
Molecules
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pfeiffer, A., Kapinsky, M., Orsó, E., Schütz, G., Schindler, H., Nagy, P., ... Schmitz, G. (2001). The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction. Single Molecules, 2(2), 113-115.

The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction. / Pfeiffer, A.; Kapinsky, M.; Orsó, E.; Schütz, G.; Schindler, H.; Nagy, P.; Rothe, G.; Szöllősi, J.; Damjanovich, S.; Schmitz, G.

In: Single Molecules, Vol. 2, No. 2, 2001, p. 113-115.

Research output: Contribution to journalArticle

Pfeiffer, A, Kapinsky, M, Orsó, E, Schütz, G, Schindler, H, Nagy, P, Rothe, G, Szöllősi, J, Damjanovich, S & Schmitz, G 2001, 'The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction', Single Molecules, vol. 2, no. 2, pp. 113-115.
Pfeiffer A, Kapinsky M, Orsó E, Schütz G, Schindler H, Nagy P et al. The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction. Single Molecules. 2001;2(2):113-115.
Pfeiffer, A. ; Kapinsky, M. ; Orsó, E. ; Schütz, G. ; Schindler, H. ; Nagy, P. ; Rothe, G. ; Szöllősi, J. ; Damjanovich, S. ; Schmitz, G. / The cholesterol content of the plasma membrane as a regulator of CD14 dependent signal transduction. In: Single Molecules. 2001 ; Vol. 2, No. 2. pp. 113-115.
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