The chaperonins of Synechocystis PCC 6803 differ in heat inducibility and chaperone activity

Eszter Kovács, Saskia M. Van Der Vies, A. Glatz, Zsolt Török, Viktória Varvasovszki, I. Horváth, L. Vígh

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The chaperonins GroEL and Cpn60 were isolated from the cyanobacterium Synechocystis PCC 6803 and characterized. In cells grown under optimal conditions their ratio was about one to one. However, the amount of GroEL increased considerably more than that of Cpn60 in response to heat stress. The labile chaperonin oligomer required stabilization by MgATP or glycerol during isolation. Use of the E. coli mutant strain, groEL44 revealed that the functional properties of the two cyanobacterial chaperonins are strikingly different. Overexpression of cyanobacterial GroEL in the E. coli mutant strain allowed growth at elevated temperature, the formation of mature bacteriophage T4, and active Rubisco enzyme assembly. In contrast, Cpn60 partially complemented the temperature-sensitive phenotype, the Rubisco assembly defect and did not promote the growth of the bacteriophage T4. The difference in chaperone activity of the two cyanobacterial chaperonins very probably reflects the unique chaperonin properties required during the life of Synechocystis PCC 6803.

Original languageEnglish
Pages (from-to)908-915
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume289
Issue number4
DOIs
Publication statusPublished - Dec 14 2001

Fingerprint

Chaperonins
Synechocystis
Hot Temperature
Ribulose-Bisphosphate Carboxylase
Bacteriophage T4
Bacteriophages
Escherichia coli
Heat-Shock Response
Temperature
Cyanobacteria
Growth
Oligomers
Glycerol
Stabilization
Adenosine Triphosphate
Cells
Phenotype
Defects
Enzymes

Keywords

  • Chaperonin purification
  • Cyanobacterium
  • GroEL
  • Heat shock
  • Synechocystis PCC 6803

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The chaperonins of Synechocystis PCC 6803 differ in heat inducibility and chaperone activity. / Kovács, Eszter; Van Der Vies, Saskia M.; Glatz, A.; Török, Zsolt; Varvasovszki, Viktória; Horváth, I.; Vígh, L.

In: Biochemical and Biophysical Research Communications, Vol. 289, No. 4, 14.12.2001, p. 908-915.

Research output: Contribution to journalArticle

Kovács, Eszter ; Van Der Vies, Saskia M. ; Glatz, A. ; Török, Zsolt ; Varvasovszki, Viktória ; Horváth, I. ; Vígh, L. / The chaperonins of Synechocystis PCC 6803 differ in heat inducibility and chaperone activity. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 289, No. 4. pp. 908-915.
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