The caspase-3 cleavage product of the plasma membrane Ca 2+-ATPase 4b is activated and appropriately targeted

Katalin Pászty, Géza Antalffy, Alan R. Penheiter, László Homolya, Rita Padányi, Attila Iliás, Adelaida G. Filoteo, John T. Penniston, Ágnes Enyedi

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22 Citations (Scopus)

Abstract

The calmodulin-activated transporter hPMCA4 (human plasma membrane Ca 2+-ATPase isoform 4) is a target for cleavage by caspase-3 during apoptosis. We have demonstrated that caspase-3 generates a 120 kDa fragment of this pump which lacks the complete autoinhibitory sequence [Paszty, Verma, Padanyi, Filoteo, Penniston and Enyedi (2002) J. Biol. Chem. 277, 6822-6829]. In the present study we analysed further the characteristics of the fragment of hPMCA4b produced by caspase-3. We did this by overexpressing the caspase-3 cleavage product of hPMCA4b in COS-7 and MDCKII (Madin-Darby canine kidney II) cells. This technique made it possible to clearly define the properties of this fragment, and we showed that it is constitutively active, as it forms a phosphoenzyme intermediate and has high Ca2+ transport activity in the absence of calmodulin. When this fragment of hPMCA4b was stably expressed in MDCKII cell clones, it was targeted without degradation to the basolateral plasma membrane. In summary, our studies emphasize that the caspase-3 cleavage product of hPMCA4b is constitutively active, and that the C-terminus is not required for proper targeting of hPMCA4b to the plasma membrane. Also, for the first time, we have generated cell clones that stably express a constitutively active PMCA.

Original languageEnglish
Pages (from-to)687-692
Number of pages6
JournalBiochemical Journal
Volume391
Issue number3
DOIs
Publication statusPublished - Nov 1 2005

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Keywords

  • Activation
  • Apoptosis
  • Calmodulin
  • Caspase-3
  • Localization
  • Plasma membrane Ca-ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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