The C-terminal domains of ADA2 proteins determine selective incorporation into GCN5-containing complexes that target histone H3 or H4 for acetylation

Edith E. Vamos, I. Boros

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

ADA2 adaptor proteins are essential subunits of GCN5-containing histone acetyltransferase (HAT) complexes. In metazoa ADA2a is present in the histone H4-specific ATAC, and ADA2b in the histone H3-specific SAGA complex. Using domain-swapped ADA2 chimeras, we determined that the in vivo function of Drosophila melanogaster SAGA and ATAC HAT complexes depend on the C-terminal region of the ADA2 subunit they contain. Our findings demonstrate that the ADA2 C-terminal regions play an important role in the specific incorporation of ADA2 into SAGA- or ATAC-type complexes, which in turn determines H3- or H4-specific histone targeting.

Original languageEnglish
Pages (from-to)3279-3286
Number of pages8
JournalFEBS Letters
Volume586
Issue number19
DOIs
Publication statusPublished - Sep 21 2012

Fingerprint

Acetylation
Histones
Histone Acetyltransferases
Adaptor Protein Complex Subunits
Proteins
Drosophila melanogaster
Protein Domains

Keywords

  • ADA2
  • ATAC complex
  • GCN5 KAT2
  • Histone acetylation
  • SAGA complex

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

The C-terminal domains of ADA2 proteins determine selective incorporation into GCN5-containing complexes that target histone H3 or H4 for acetylation. / E. Vamos, Edith; Boros, I.

In: FEBS Letters, Vol. 586, No. 19, 21.09.2012, p. 3279-3286.

Research output: Contribution to journalArticle

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