The bluetail transposon: Evidence for independent cis-regulatory domains and domain boundaries in the bithorax complex

M. Galloni, H. Gyurkovics, P. Schedl, F. Karch

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

An extremely large cis-regulatory region generates the parasegment-specific expression patterns of the homeotic genes in the bithorax complex. We present evidence supporting the idea that this cis-regulatory region is subdivided into independent cis-regulatory domains. We describe a Ubx-lacZ transposon which is inserted into one of these domains, iab-7. The PS12-specific pattern of LacZ expression from this reporter indicates that it is subject to the control of the iab-7 cis-regulatory domain, but is protected from the effects of adjacent regulatory domains. Protection on the proximal side appears to be provided by the Fab-7 boundary element. Deletion of this boundary results in the ectopic activation of iab-7 in PS11 (where the iab-6 cis-regulatory domain normally functions). We show that the Fab-7 boundary, like other boundaries, has an unusual chromatin structure.

Original languageEnglish
Pages (from-to)1087-1097
Number of pages11
JournalEMBO Journal
Volume12
Issue number3
DOIs
Publication statusPublished - 1993

Keywords

  • Chromatin
  • Cis-regulatory domain
  • Domain boundary
  • Drosophila
  • Homeotic

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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