The blocking effect of PGE2 on lymphocyte reactivity in vitro is influenced by high concentrations of human serum

Research output: Contribution to journalArticle

Abstract

PGE2 solubilized in human serum as well as dialyzed or heat inactivated human serum was tested for modulating natural cytotoxicity as compared to PGE2 solubilized in culture medium. PGE2 dissolved in human serum failed to affect cytotoxicity, while that solubilized in dialyzed or heat inactivated serum suppressed in vitro cytotoxic activity of the lymphocytes similarly to PGE2 in culture medium. It is concluded that the immunomodulatory potential of PGE2 observed in vitro is inhibited by high concentrations of human serum and that in vitro findings with this compound might not reflect the situation in vivo.

Original languageEnglish
Pages (from-to)83-85
Number of pages3
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume32
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

Lymphocytes
Dinoprostone
Serum
Cytotoxicity
Culture Media
Hot Temperature
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

@article{0958402dfb98456dbbbdcb49ca697549,
title = "The blocking effect of PGE2 on lymphocyte reactivity in vitro is influenced by high concentrations of human serum",
abstract = "PGE2 solubilized in human serum as well as dialyzed or heat inactivated human serum was tested for modulating natural cytotoxicity as compared to PGE2 solubilized in culture medium. PGE2 dissolved in human serum failed to affect cytotoxicity, while that solubilized in dialyzed or heat inactivated serum suppressed in vitro cytotoxic activity of the lymphocytes similarly to PGE2 in culture medium. It is concluded that the immunomodulatory potential of PGE2 observed in vitro is inhibited by high concentrations of human serum and that in vitro findings with this compound might not reflect the situation in vivo.",
author = "J. Szekeres-Barth{\'o} and P. Varga",
year = "1988",
doi = "10.1016/0952-3278(88)90100-7",
language = "English",
volume = "32",
pages = "83--85",
journal = "Prostaglandins Leukotrienes and Essential Fatty Acids",
issn = "0952-3278",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - The blocking effect of PGE2 on lymphocyte reactivity in vitro is influenced by high concentrations of human serum

AU - Szekeres-Barthó, J.

AU - Varga, P.

PY - 1988

Y1 - 1988

N2 - PGE2 solubilized in human serum as well as dialyzed or heat inactivated human serum was tested for modulating natural cytotoxicity as compared to PGE2 solubilized in culture medium. PGE2 dissolved in human serum failed to affect cytotoxicity, while that solubilized in dialyzed or heat inactivated serum suppressed in vitro cytotoxic activity of the lymphocytes similarly to PGE2 in culture medium. It is concluded that the immunomodulatory potential of PGE2 observed in vitro is inhibited by high concentrations of human serum and that in vitro findings with this compound might not reflect the situation in vivo.

AB - PGE2 solubilized in human serum as well as dialyzed or heat inactivated human serum was tested for modulating natural cytotoxicity as compared to PGE2 solubilized in culture medium. PGE2 dissolved in human serum failed to affect cytotoxicity, while that solubilized in dialyzed or heat inactivated serum suppressed in vitro cytotoxic activity of the lymphocytes similarly to PGE2 in culture medium. It is concluded that the immunomodulatory potential of PGE2 observed in vitro is inhibited by high concentrations of human serum and that in vitro findings with this compound might not reflect the situation in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0024005323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024005323&partnerID=8YFLogxK

U2 - 10.1016/0952-3278(88)90100-7

DO - 10.1016/0952-3278(88)90100-7

M3 - Article

VL - 32

SP - 83

EP - 85

JO - Prostaglandins Leukotrienes and Essential Fatty Acids

JF - Prostaglandins Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 2

ER -