The bimodal quasi-static and dynamic elastance of the murine lung

Graeme R. Zosky, Tibor Z. Janosi, A. Adamicza, Elizabeth M. Bozanich, Vincenzo Cannizzaro, Alexander N. Larcombe, Debra J. Turner, Peter D. Sly, Z. Hantos

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The double sigmoidal nature of the mouse pressure-volume (PV) curve is well recognized but largely ignored. This study systematically examined the effect of inflating the mouse lung to 40 cm H2O transrespiratory pressure (Prs) in vivo. Adult BALB/c mice were anesthetized, tracheostomized, and mechanically ventilated. Thoracic gas volume was calculated using plethysmography and electrical stimulation of the intercostal muscles. Lung mechanics were tracked during inflation-deflation maneuvers using a modification of the forced oscillation technique. Inflation beyond 20 cm H2O caused a shift in subsequent PV curves with an increase in slope of the inflation limb and an increase in lung volume at 20 cm H2O. There was an overall decrease in tissue elastance and a fundamental change in its volume dependence. This apparent "softening" of the lung could be recovered by partial degassing of the lung or applying a negative transrespiratory pressure such that lung volume decreased below functional residual capacity. Allowing the lung to spontaneously recover revealed that the lung required ∼1 h of mechanical ventilation to return to the original state. We propose a number of possible mechanisms for these observations and suggest that they are most likely explained by the unfolding of alveolar septa and the subsequent redistribution of the fluid lining the alveoli at high transrespiratory pressure.

Original languageEnglish
Pages (from-to)685-692
Number of pages8
JournalJournal of Applied Physiology
Volume105
Issue number2
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Lung
Economic Inflation
Pressure
Intercostal Muscles
Functional Residual Capacity
Plethysmography
Mechanics
Artificial Respiration
Electric Stimulation
Thorax
Extremities
Gases

Keywords

  • Forced oscillation technique
  • Lung softening
  • Mouse
  • Pressure-volume curve
  • Total lung capacity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

The bimodal quasi-static and dynamic elastance of the murine lung. / Zosky, Graeme R.; Janosi, Tibor Z.; Adamicza, A.; Bozanich, Elizabeth M.; Cannizzaro, Vincenzo; Larcombe, Alexander N.; Turner, Debra J.; Sly, Peter D.; Hantos, Z.

In: Journal of Applied Physiology, Vol. 105, No. 2, 08.2008, p. 685-692.

Research output: Contribution to journalArticle

Zosky, GR, Janosi, TZ, Adamicza, A, Bozanich, EM, Cannizzaro, V, Larcombe, AN, Turner, DJ, Sly, PD & Hantos, Z 2008, 'The bimodal quasi-static and dynamic elastance of the murine lung', Journal of Applied Physiology, vol. 105, no. 2, pp. 685-692. https://doi.org/10.1152/japplphysiol.90328.2008
Zosky, Graeme R. ; Janosi, Tibor Z. ; Adamicza, A. ; Bozanich, Elizabeth M. ; Cannizzaro, Vincenzo ; Larcombe, Alexander N. ; Turner, Debra J. ; Sly, Peter D. ; Hantos, Z. / The bimodal quasi-static and dynamic elastance of the murine lung. In: Journal of Applied Physiology. 2008 ; Vol. 105, No. 2. pp. 685-692.
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