The axon arbourisation of nuclei isthmi neurons in the optic tectum of the chick and pigeon. A Golgi and anterograde tracer-study

T. Tömböl, M. D. Eyre, A. Alpár, A. Németh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The optic tectum is reciprocally connected to the nuclei isthmi pars magnocellularis (Imc) and pars parvocellularis (Ipc), which have different modulatory effects on optic transmission. We studied the axon arbourisation of these isthmic nuclei in the optic tectum in order to differentiate between them using Golgi-impregnated preparations both in chickens and pigeons. In addition, sections from animals injected with the anterograde tracer biotinylated dextran-amine (BDA) into the Imc were examined in the bright-field and electron microscope to identify the axon arbourisations and terminals. Also, GABA immunogold stained sections were examined in the electron microscope. In Golgi preparations, slab-like (or poplar tree-like) axon terminal arbourisations of both magnocellular and parvocellular isthmic nuclei neurons were found extending to the tectal surface, with similar branching patterns, but different lengths. The axon arbourisations extending from layer 5 of the optic tectum to the surface were termed type 1, whereas those extending from the internal (12-11) layers to the tectal surface were termed type 2. Type 2 arbourisations very closely matched arbourisations observed in BDA injected material, indicating that Imc neurons gave rise to type 2 arbourisations. The two kinds of axon arbourisation in the external tectal layers were alike in both types of bird, except for the width, which was about 10 μm larger in the type 2 axon arbour. Controlling for size, there was no significant difference between chicks and pigeons. The significance of these afferents in the optic tectum is discussed.

Original languageEnglish
Pages (from-to)371-380
Number of pages10
JournalAnatomy and Embryology
Volume209
Issue number5
DOIs
Publication statusPublished - Jul 2005

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Superior Colliculi
Columbidae
Axons
Neurons
Presynaptic Terminals
Electrons
gamma-Aminobutyric Acid
Birds
Chickens
biotinylated dextran amine

Keywords

  • Axon terminals
  • BDA anterograde tracer
  • GABA Immunogold labeling
  • Golgi
  • Nuclei isthmi

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

The axon arbourisation of nuclei isthmi neurons in the optic tectum of the chick and pigeon. A Golgi and anterograde tracer-study. / Tömböl, T.; Eyre, M. D.; Alpár, A.; Németh, A.

In: Anatomy and Embryology, Vol. 209, No. 5, 07.2005, p. 371-380.

Research output: Contribution to journalArticle

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