The association between angiotensin II-induced free radical generation and membrane fluidity in neutrophils of patients with metabolic syndrome

Ildikó Seres, Gabriella Fóris, Zsuzsa Varga, Béla Kosztáczky, Andrea Kassai, Zoltán Balogh, Péter Fülöp, György Paragh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Angiotensin II (Ang II) is able to induce free radical generation in neutrophils, which is more elevated in neutrophils of patients with hypercholesterolemia (HC). In addition, the signal processing through angiotensin I (Ang I) receptors is altered. In present study, we compared the Ang II-triggered free radical generation of neutrophils obtained from patients with relatively isolated forms of metabolic syndrome (MS) with membrane-bound cholesterol content and membrane fluidity. We determined the enhancement of Ang II-induced superoxide anion and leukotriene C4 (LTC4) generation, membrane fluidity and cell-bound cholesterol content of neutrophils obtained from 12 control subjects, 11 patients with obesity (Ob), 10 patients with type 2 diabetes mellitus (t2-DM) and 12 patients with HC. The alteration of signal processing was studied after preincubation with different inhibiting drugs. Superoxide anion, LTC4 production and membrane rigidity were increased in the following order: control < Ob < t2-DM < HC. Both Ang II-induced superoxide anion and LTC4 generation were decreased in control cells by pertussis toxin and fluvastatin (Flu), whereas in each patient group, mepacrin, verapamil and Flu were effective, suggesting alterations in signal pathways, which may be attributed to isoprenylation. The enhancement of superoxide anion and LTC4 generation correlated significantly with membrane rigidity, independently from the experimental groups and membrane-bound cholesterol content. Membrane rigidity of neutrophils, obtained from patients with MS, plays a role in Ang II-induced free radical generation independent of intracellular cholesterol homeostasis.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalJournal of Membrane Biology
Volume214
Issue number1-2
DOIs
Publication statusPublished - Nov 1 2006

Keywords

  • Angiotensin II
  • Free radical
  • Membrane fluidity
  • Metabolic syndrome
  • Neutrophil

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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