The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle

M. Flipphi, Nathalie Oestreicher, Valérie Nicolas, Audrey Guitton, Christian Vélot

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In Aspergillus nidulans, the utilization of acetate as sole carbon source requires several genes (acu). Most of them are also required for the utilization of fatty acids. This is the case for acuD and acuE, which encode the two glyoxylate cycle-specific enzymes, isocitrate lyase and malate synthase, respectively, but also for acuL that we have identified as AN7287, and characterized in this study. Deletion of acuL resulted in the same phenotype as the original acuL217 mutant. acuL encodes a 322-amino acid protein which displays all the structural features of a mitochondrial membrane carrier, and shares 60% identity with the Saccharomyces cerevisiae succinate/fumarate mitochondrial antiporter Sfc1p (also named Acr1p). Consistently, the AcuL protein was shown to localize in mitochondria, and partial cross-complementation was observed between the S. cerevisiae and A. nidulans homologues. Extensive phenotypic characterization suggested that the acuL gene is involved in the utilization of carbon sources that are catabolized via the TCA cycle, and therefore require gluconeogenesis. In addition, acuL proves to be co-regulated with acuD and acuE. Overall, our data suggest that AcuL could link the glyoxylate cycle to gluconeogenesis by exchanging cytoplasmic succinate for mitochondrial fumarate.

Original languageEnglish
Pages (from-to)9-22
Number of pages14
JournalFungal Genetics and Biology
Volume68
DOIs
Publication statusPublished - 2014

Fingerprint

Aspergillus nidulans
Fumarates
Gluconeogenesis
Succinic Acid
Saccharomyces cerevisiae
Malate Synthase
Carbon
Isocitrate Lyase
Antiporters
Mitochondrial Membranes
Genes
Mitochondria
Proteins
Acetates
Fatty Acids
Phenotype
Amino Acids
Enzymes
glyoxylic acid

Keywords

  • Aspergillus nidulans
  • Cross-complementation
  • Gluconeogenesis
  • Glyoxylate pathway
  • Succinate-fumarate antiport
  • TCA cycle

ASJC Scopus subject areas

  • Genetics
  • Microbiology
  • Medicine(all)

Cite this

The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle. / Flipphi, M.; Oestreicher, Nathalie; Nicolas, Valérie; Guitton, Audrey; Vélot, Christian.

In: Fungal Genetics and Biology, Vol. 68, 2014, p. 9-22.

Research output: Contribution to journalArticle

@article{d334766b38994bd1bd396710ea44d193,
title = "The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle",
abstract = "In Aspergillus nidulans, the utilization of acetate as sole carbon source requires several genes (acu). Most of them are also required for the utilization of fatty acids. This is the case for acuD and acuE, which encode the two glyoxylate cycle-specific enzymes, isocitrate lyase and malate synthase, respectively, but also for acuL that we have identified as AN7287, and characterized in this study. Deletion of acuL resulted in the same phenotype as the original acuL217 mutant. acuL encodes a 322-amino acid protein which displays all the structural features of a mitochondrial membrane carrier, and shares 60{\%} identity with the Saccharomyces cerevisiae succinate/fumarate mitochondrial antiporter Sfc1p (also named Acr1p). Consistently, the AcuL protein was shown to localize in mitochondria, and partial cross-complementation was observed between the S. cerevisiae and A. nidulans homologues. Extensive phenotypic characterization suggested that the acuL gene is involved in the utilization of carbon sources that are catabolized via the TCA cycle, and therefore require gluconeogenesis. In addition, acuL proves to be co-regulated with acuD and acuE. Overall, our data suggest that AcuL could link the glyoxylate cycle to gluconeogenesis by exchanging cytoplasmic succinate for mitochondrial fumarate.",
keywords = "Aspergillus nidulans, Cross-complementation, Gluconeogenesis, Glyoxylate pathway, Succinate-fumarate antiport, TCA cycle",
author = "M. Flipphi and Nathalie Oestreicher and Val{\'e}rie Nicolas and Audrey Guitton and Christian V{\'e}lot",
year = "2014",
doi = "10.1016/j.fgb.2014.04.012",
language = "English",
volume = "68",
pages = "9--22",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - The Aspergillus nidulans acuL gene encodes a mitochondrial carrier required for the utilization of carbon sources that are metabolized via the TCA cycle

AU - Flipphi, M.

AU - Oestreicher, Nathalie

AU - Nicolas, Valérie

AU - Guitton, Audrey

AU - Vélot, Christian

PY - 2014

Y1 - 2014

N2 - In Aspergillus nidulans, the utilization of acetate as sole carbon source requires several genes (acu). Most of them are also required for the utilization of fatty acids. This is the case for acuD and acuE, which encode the two glyoxylate cycle-specific enzymes, isocitrate lyase and malate synthase, respectively, but also for acuL that we have identified as AN7287, and characterized in this study. Deletion of acuL resulted in the same phenotype as the original acuL217 mutant. acuL encodes a 322-amino acid protein which displays all the structural features of a mitochondrial membrane carrier, and shares 60% identity with the Saccharomyces cerevisiae succinate/fumarate mitochondrial antiporter Sfc1p (also named Acr1p). Consistently, the AcuL protein was shown to localize in mitochondria, and partial cross-complementation was observed between the S. cerevisiae and A. nidulans homologues. Extensive phenotypic characterization suggested that the acuL gene is involved in the utilization of carbon sources that are catabolized via the TCA cycle, and therefore require gluconeogenesis. In addition, acuL proves to be co-regulated with acuD and acuE. Overall, our data suggest that AcuL could link the glyoxylate cycle to gluconeogenesis by exchanging cytoplasmic succinate for mitochondrial fumarate.

AB - In Aspergillus nidulans, the utilization of acetate as sole carbon source requires several genes (acu). Most of them are also required for the utilization of fatty acids. This is the case for acuD and acuE, which encode the two glyoxylate cycle-specific enzymes, isocitrate lyase and malate synthase, respectively, but also for acuL that we have identified as AN7287, and characterized in this study. Deletion of acuL resulted in the same phenotype as the original acuL217 mutant. acuL encodes a 322-amino acid protein which displays all the structural features of a mitochondrial membrane carrier, and shares 60% identity with the Saccharomyces cerevisiae succinate/fumarate mitochondrial antiporter Sfc1p (also named Acr1p). Consistently, the AcuL protein was shown to localize in mitochondria, and partial cross-complementation was observed between the S. cerevisiae and A. nidulans homologues. Extensive phenotypic characterization suggested that the acuL gene is involved in the utilization of carbon sources that are catabolized via the TCA cycle, and therefore require gluconeogenesis. In addition, acuL proves to be co-regulated with acuD and acuE. Overall, our data suggest that AcuL could link the glyoxylate cycle to gluconeogenesis by exchanging cytoplasmic succinate for mitochondrial fumarate.

KW - Aspergillus nidulans

KW - Cross-complementation

KW - Gluconeogenesis

KW - Glyoxylate pathway

KW - Succinate-fumarate antiport

KW - TCA cycle

UR - http://www.scopus.com/inward/record.url?scp=84901504387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901504387&partnerID=8YFLogxK

U2 - 10.1016/j.fgb.2014.04.012

DO - 10.1016/j.fgb.2014.04.012

M3 - Article

C2 - 24835019

AN - SCOPUS:84901504387

VL - 68

SP - 9

EP - 22

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

ER -