The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching

Tina Reiner, Caroline Hoefle, Christina Huesmann, Dalma Ménesi, A. Fehér, Ralph Hückelhoven

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Key message: TheArabidopsisreceptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction.

Abstract: Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like plant growth, development and immune responses. Recent studies suggest that RLCKs of the VI_A subfamily are possible downstream effectors of the small monomeric G proteins of the plant-specific Rho family, called ‘Rho of plants’ (RAC/ROPs). Here, we describe Arabidopsisthaliana AtRLCK VI_A3 as a molecular interactor of AtROPs. In Arabidopsis epidermal cells, transient co-expression of plasma membrane located constitutively activated (CA) AtROP4 or CA AtROP6 resulting in the recruitment of green fluorescent protein-tagged AtRLCK VI_A3 to the cell periphery. Intrinsic kinase activity of AtRLCK VI_A3 was enhanced in the presence of CA AtROP6 in vitro and further suggested a functional interaction between the proteins. In the interaction of the biotrophic powdery mildew fungus Erysiphe cruciferarum (E. cruciferarum) and its host plant Arabidopsis, Atrlck VI_A3 mutant lines supported enhanced fungal reproduction. Furthermore Atrlck VI_A3 mutant lines showed slightly reduced size and an increase in trichome branch number compared to wild-type plants. In summary, our data suggest a role of the AtROP-regulated AtRLCK VI_A3 in basal resistance to E. cruciferarum as well as in plant growth and cellular differentiation during trichome morphogenesis. Results are discussed in the context of literature suggesting a function of RAC/ROPs in both resistance and susceptibility to pathogen infection.

Original languageEnglish
Pages (from-to)457-468
Number of pages12
JournalPlant Cell Reports
Volume34
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

powdery mildew
trichomes
branching
phosphotransferases (kinases)
Arabidopsis
Erysiphe cruciferarum
receptors
plant growth
mutants
pathogens
G-proteins
green fluorescent protein
morphogenesis
plasma membrane
host plants
immune response
cells
fungi
infection
proteins

Keywords

  • Arabidopsis thaliana
  • Erysiphe cruciferarum
  • RAC/ROP GTPase
  • Receptor-like cytoplasmic kinase (RLCK)
  • Trichome

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching. / Reiner, Tina; Hoefle, Caroline; Huesmann, Christina; Ménesi, Dalma; Fehér, A.; Hückelhoven, Ralph.

In: Plant Cell Reports, Vol. 34, No. 3, 2014, p. 457-468.

Research output: Contribution to journalArticle

Reiner, Tina ; Hoefle, Caroline ; Huesmann, Christina ; Ménesi, Dalma ; Fehér, A. ; Hückelhoven, Ralph. / The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching. In: Plant Cell Reports. 2014 ; Vol. 34, No. 3. pp. 457-468.
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T1 - The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching

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AU - Hoefle, Caroline

AU - Huesmann, Christina

AU - Ménesi, Dalma

AU - Fehér, A.

AU - Hückelhoven, Ralph

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AB - Key message: TheArabidopsisreceptor-like cytoplasmic kinase AtRLCK VI_A3 is activated by AtROPs and is involved in trichome branching and pathogen interaction.Abstract: Receptor-like cytoplasmic kinases (RLCKs) belong to the large superfamily of receptor-like kinases, which are involved in a variety of cellular processes like plant growth, development and immune responses. Recent studies suggest that RLCKs of the VI_A subfamily are possible downstream effectors of the small monomeric G proteins of the plant-specific Rho family, called ‘Rho of plants’ (RAC/ROPs). Here, we describe Arabidopsisthaliana AtRLCK VI_A3 as a molecular interactor of AtROPs. In Arabidopsis epidermal cells, transient co-expression of plasma membrane located constitutively activated (CA) AtROP4 or CA AtROP6 resulting in the recruitment of green fluorescent protein-tagged AtRLCK VI_A3 to the cell periphery. Intrinsic kinase activity of AtRLCK VI_A3 was enhanced in the presence of CA AtROP6 in vitro and further suggested a functional interaction between the proteins. In the interaction of the biotrophic powdery mildew fungus Erysiphe cruciferarum (E. cruciferarum) and its host plant Arabidopsis, Atrlck VI_A3 mutant lines supported enhanced fungal reproduction. Furthermore Atrlck VI_A3 mutant lines showed slightly reduced size and an increase in trichome branch number compared to wild-type plants. In summary, our data suggest a role of the AtROP-regulated AtRLCK VI_A3 in basal resistance to E. cruciferarum as well as in plant growth and cellular differentiation during trichome morphogenesis. Results are discussed in the context of literature suggesting a function of RAC/ROPs in both resistance and susceptibility to pathogen infection.

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