The Arabidopsis Anaphase-Promoting Complex or Cyclosome: Molecular and Genetic Characterization of the APC2 Subunit

Arnaud Capron, Olivier Serralbo, Katalin Fülöp, Florian Frugier, Yves Parmentier, Aiwu Dong, Alain Lecureuil, Philippe Guerche, É. Kondorosi, Ben Scheres, Pascal Genschik

Research output: Contribution to journalArticle

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Abstract

In yeast and animals, the anaphase-promoting complex or cyclosome (APC/C) is an essential ubiquitin protein ligase that regulates mitotic progression and exit by controlling the stability of cell cycle regulatory proteins, such as securin and the mitotic cyclins. In plants, the function, regulation, and substrates of the APC/C are poorly understood. To gain more insight into the roles of the plant APC/C, we characterized at the molecular level one of its subunits, APC2, which is encoded by a single-copy gene in Arabidopsis. We show that the Arabidopsis gene is able to partially complement a budding yeast apc2 ts mutant. By yeast two-hybrid assays, we demonstrate an interaction of APC2 with two other APC/C subunits: APC11 and APC8/CDC23. A reverse-genetic approach identified Arabidopsis plants carrying T-DNA insertions in the APC2 gene. apc2 null mutants are impaired in female megagametogenesis and accumulate a cyclin-β-glucuronidase reporter protein but do not display metaphase arrest, as observed in other systems. The APC2 gene is expressed in various plant organs and does not seem to be cell cycle regulated. Finally, we report intriguing differences in APC2 protein subcellular localization compared with that in other systems. Our observations support a conserved function of the APC/C in plants but a different mode of regulation.

Original languageEnglish
Pages (from-to)2370-2382
Number of pages13
JournalPlant Cell
Volume15
Issue number10
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Anaphase-Promoting Complex-Cyclosome
Arabidopsis
Molecular Biology
Genes
Yeast
Cyclins
cyclins
cell cycle
Securin
genes
yeasts
Reverse Genetics
two hybrid system techniques
mutants
Cell Cycle Proteins
Two-Hybrid System Techniques
ubiquitin-protein ligase
Saccharomycetales
Ubiquitin-Protein Ligases
Glucuronidase

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Capron, A., Serralbo, O., Fülöp, K., Frugier, F., Parmentier, Y., Dong, A., ... Genschik, P. (2003). The Arabidopsis Anaphase-Promoting Complex or Cyclosome: Molecular and Genetic Characterization of the APC2 Subunit. Plant Cell, 15(10), 2370-2382. https://doi.org/10.1105/tpc.013847

The Arabidopsis Anaphase-Promoting Complex or Cyclosome : Molecular and Genetic Characterization of the APC2 Subunit. / Capron, Arnaud; Serralbo, Olivier; Fülöp, Katalin; Frugier, Florian; Parmentier, Yves; Dong, Aiwu; Lecureuil, Alain; Guerche, Philippe; Kondorosi, É.; Scheres, Ben; Genschik, Pascal.

In: Plant Cell, Vol. 15, No. 10, 10.2003, p. 2370-2382.

Research output: Contribution to journalArticle

Capron, A, Serralbo, O, Fülöp, K, Frugier, F, Parmentier, Y, Dong, A, Lecureuil, A, Guerche, P, Kondorosi, É, Scheres, B & Genschik, P 2003, 'The Arabidopsis Anaphase-Promoting Complex or Cyclosome: Molecular and Genetic Characterization of the APC2 Subunit', Plant Cell, vol. 15, no. 10, pp. 2370-2382. https://doi.org/10.1105/tpc.013847
Capron, Arnaud ; Serralbo, Olivier ; Fülöp, Katalin ; Frugier, Florian ; Parmentier, Yves ; Dong, Aiwu ; Lecureuil, Alain ; Guerche, Philippe ; Kondorosi, É. ; Scheres, Ben ; Genschik, Pascal. / The Arabidopsis Anaphase-Promoting Complex or Cyclosome : Molecular and Genetic Characterization of the APC2 Subunit. In: Plant Cell. 2003 ; Vol. 15, No. 10. pp. 2370-2382.
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