The applicability of large-scale sperm cryopreservation in wels catfish (Silurus glanis) optimized for hatchery practice

Zoltán Bokor, Gergely Bernáth, Levente Várkonyi, József Molnár, Zete Levente Láng, Zsófia Tarnai-Király, Enikő Solymosi, B. Urbányi

Research output: Contribution to journalArticle

Abstract

In the presented study, effects of sperm cryopreservation methods (Polystyrene box-P. box vs a controlled-rate freezer-CRF) and various semen confection methods during freezing (5 mL straw vs. 10 mL cryotube) on sperm motility parameters and fertilization capacity of wels catfish (Silurus glanis) were tested. In general, pMOT (Control: 89 ± 3%, Straw P. box: 50 ± 9%, Straw CRF: 53 ± 12%, Cryotube: 52 ± 7%) and BCF (Control: 30 ± 1 Hz, Straw P. box: 27 ± 1 Hz, Straw CRF: 26 ± 1 Hz, Cryotube: 27 ± 1 Hz) showed significant reduction following thawing. ALH reduced significantly in the case of the straw frozen with P. box (1.2 ± 0.2 μm) compared to the fresh control (1.5 ± 0.1 μm). However, VCL (Control: 108 ± 6 μm/s, Straw P. box: 107 ± 5 μm/s, Straw CRF: 108 ± 8 μm/s, Cryotube: 109 ± 7 μm/s) and VSL (Control: 94 ± 6 μm/s, Straw P. box: 99 ± 4 μm/s, Straw CRF: 99 ± 9 μm/s, Cryotube: 99 ± 7 μm/s) did not decrease. A significantly higher LIN was measured using the straw cryopreserved in the P. box (92 ± 2%) and the CRF (91 ± 2%) in comparison with the fresh control (86 ± 1%). No significant difference in the fertilization capacity when eggs were fertilized by frozen/thawed (Straw P. box: 75 ± 5%, Straw CRF: 72 ± 3%, Cryotube: 66 ± 6%) or fresh (control, 68 ± 4%) semen was observed (P >.05). As well, no significant correlation between none of sperm motility parameters tested and the fertilization rate were recorded (P >.05). All tested sperm cryopreservation showed a similar high efficiency in the hatchery practice of wels catfis.

Original languageEnglish
Pages (from-to)337-340
Number of pages4
JournalAquaculture
Volume506
DOIs
Publication statusPublished - May 15 2019

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Silurus glanis
cryopreservation
hatchery
sperm
straw
hatcheries
spermatozoa
fertilization (reproduction)
motility
sperm motility
semen
Allan Hills meteorite
freezers
polystyrenes
thawing

Keywords

  • Cryopreservation
  • Large-scale
  • Motility assessment
  • Sperm
  • Wels catfish

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The applicability of large-scale sperm cryopreservation in wels catfish (Silurus glanis) optimized for hatchery practice. / Bokor, Zoltán; Bernáth, Gergely; Várkonyi, Levente; Molnár, József; Láng, Zete Levente; Tarnai-Király, Zsófia; Solymosi, Enikő; Urbányi, B.

In: Aquaculture, Vol. 506, 15.05.2019, p. 337-340.

Research output: Contribution to journalArticle

Bokor, Zoltán ; Bernáth, Gergely ; Várkonyi, Levente ; Molnár, József ; Láng, Zete Levente ; Tarnai-Király, Zsófia ; Solymosi, Enikő ; Urbányi, B. / The applicability of large-scale sperm cryopreservation in wels catfish (Silurus glanis) optimized for hatchery practice. In: Aquaculture. 2019 ; Vol. 506. pp. 337-340.
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AU - Molnár, József

AU - Láng, Zete Levente

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AU - Urbányi, B.

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N2 - In the presented study, effects of sperm cryopreservation methods (Polystyrene box-P. box vs a controlled-rate freezer-CRF) and various semen confection methods during freezing (5 mL straw vs. 10 mL cryotube) on sperm motility parameters and fertilization capacity of wels catfish (Silurus glanis) were tested. In general, pMOT (Control: 89 ± 3%, Straw P. box: 50 ± 9%, Straw CRF: 53 ± 12%, Cryotube: 52 ± 7%) and BCF (Control: 30 ± 1 Hz, Straw P. box: 27 ± 1 Hz, Straw CRF: 26 ± 1 Hz, Cryotube: 27 ± 1 Hz) showed significant reduction following thawing. ALH reduced significantly in the case of the straw frozen with P. box (1.2 ± 0.2 μm) compared to the fresh control (1.5 ± 0.1 μm). However, VCL (Control: 108 ± 6 μm/s, Straw P. box: 107 ± 5 μm/s, Straw CRF: 108 ± 8 μm/s, Cryotube: 109 ± 7 μm/s) and VSL (Control: 94 ± 6 μm/s, Straw P. box: 99 ± 4 μm/s, Straw CRF: 99 ± 9 μm/s, Cryotube: 99 ± 7 μm/s) did not decrease. A significantly higher LIN was measured using the straw cryopreserved in the P. box (92 ± 2%) and the CRF (91 ± 2%) in comparison with the fresh control (86 ± 1%). No significant difference in the fertilization capacity when eggs were fertilized by frozen/thawed (Straw P. box: 75 ± 5%, Straw CRF: 72 ± 3%, Cryotube: 66 ± 6%) or fresh (control, 68 ± 4%) semen was observed (P >.05). As well, no significant correlation between none of sperm motility parameters tested and the fertilization rate were recorded (P >.05). All tested sperm cryopreservation showed a similar high efficiency in the hatchery practice of wels catfis.

AB - In the presented study, effects of sperm cryopreservation methods (Polystyrene box-P. box vs a controlled-rate freezer-CRF) and various semen confection methods during freezing (5 mL straw vs. 10 mL cryotube) on sperm motility parameters and fertilization capacity of wels catfish (Silurus glanis) were tested. In general, pMOT (Control: 89 ± 3%, Straw P. box: 50 ± 9%, Straw CRF: 53 ± 12%, Cryotube: 52 ± 7%) and BCF (Control: 30 ± 1 Hz, Straw P. box: 27 ± 1 Hz, Straw CRF: 26 ± 1 Hz, Cryotube: 27 ± 1 Hz) showed significant reduction following thawing. ALH reduced significantly in the case of the straw frozen with P. box (1.2 ± 0.2 μm) compared to the fresh control (1.5 ± 0.1 μm). However, VCL (Control: 108 ± 6 μm/s, Straw P. box: 107 ± 5 μm/s, Straw CRF: 108 ± 8 μm/s, Cryotube: 109 ± 7 μm/s) and VSL (Control: 94 ± 6 μm/s, Straw P. box: 99 ± 4 μm/s, Straw CRF: 99 ± 9 μm/s, Cryotube: 99 ± 7 μm/s) did not decrease. A significantly higher LIN was measured using the straw cryopreserved in the P. box (92 ± 2%) and the CRF (91 ± 2%) in comparison with the fresh control (86 ± 1%). No significant difference in the fertilization capacity when eggs were fertilized by frozen/thawed (Straw P. box: 75 ± 5%, Straw CRF: 72 ± 3%, Cryotube: 66 ± 6%) or fresh (control, 68 ± 4%) semen was observed (P >.05). As well, no significant correlation between none of sperm motility parameters tested and the fertilization rate were recorded (P >.05). All tested sperm cryopreservation showed a similar high efficiency in the hatchery practice of wels catfis.

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