The antifungal protein AFP secreted by Aspergillus giganteus does not cause detrimental effects on certain mammalian cells

Henrietta Szappanos, G. Szigeti, B. Pál, Zoltán Rusznák, G. Szücs, E. Rajnavolgyi, J. Balla, G. Balla, E. Nagy, E. Leiter, I. Pócsi, Silke Hagen, Vera Meyer, L. Csernoch

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The antifungal protein AFP is a small, cystein-rich protein secreted by the imperfect ascomycete Aspergillus giganteus. The protein efficiently inhibits the growth of filamentous fungi, including a variety of serious human and plant pathogens mainly of the genera Aspergillus and Fusarium, whereas AFP does not affect the growth of yeast and bacteria. This restricted susceptibility range makes it very attractive for medical or biotechnological use to combat fungal infection and contamination. We, therefore, analyzed whether AFP affects the growth or function of a number of mammalian cells. Here we show that the protein neither provokes any cytotoxic effects on human endothelial cells isolated from the umbilical vein nor activates the immune system. Moreover, potassium currents of neurons and astrocytes do not change in the presence of AFP and neither excitatory processes nor the intracellular calcium homeostasis of cultured skeletal muscle myotubes are affected by AFP. Our data, therefore, suggest that AFP is indeed a promising candidate for the therapeutic or biotechnological use as a potential antifungal agent.

Original languageEnglish
Pages (from-to)1717-1725
Number of pages9
JournalPeptides
Volume27
Issue number7
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Aspergillus
Cells
Proteins
Growth
Ascomycota
Mycoses
Antifungal Agents
Immune system
Skeletal Muscle Fibers
Endothelial cells
Human Umbilical Vein Endothelial Cells
Fusarium
Pathogens
Fungi
Astrocytes
Yeast
Neurons
Muscle
Immune System
Bacteria

Keywords

  • Antifungal protein (AFP)
  • Aspergillus giganteus
  • Calcium homeostasis
  • Cytotoxicity
  • Inflammatory action
  • Potassium current

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

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title = "The antifungal protein AFP secreted by Aspergillus giganteus does not cause detrimental effects on certain mammalian cells",
abstract = "The antifungal protein AFP is a small, cystein-rich protein secreted by the imperfect ascomycete Aspergillus giganteus. The protein efficiently inhibits the growth of filamentous fungi, including a variety of serious human and plant pathogens mainly of the genera Aspergillus and Fusarium, whereas AFP does not affect the growth of yeast and bacteria. This restricted susceptibility range makes it very attractive for medical or biotechnological use to combat fungal infection and contamination. We, therefore, analyzed whether AFP affects the growth or function of a number of mammalian cells. Here we show that the protein neither provokes any cytotoxic effects on human endothelial cells isolated from the umbilical vein nor activates the immune system. Moreover, potassium currents of neurons and astrocytes do not change in the presence of AFP and neither excitatory processes nor the intracellular calcium homeostasis of cultured skeletal muscle myotubes are affected by AFP. Our data, therefore, suggest that AFP is indeed a promising candidate for the therapeutic or biotechnological use as a potential antifungal agent.",
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T1 - The antifungal protein AFP secreted by Aspergillus giganteus does not cause detrimental effects on certain mammalian cells

AU - Szappanos, Henrietta

AU - Szigeti, G.

AU - Pál, B.

AU - Rusznák, Zoltán

AU - Szücs, G.

AU - Rajnavolgyi, E.

AU - Balla, J.

AU - Balla, G.

AU - Nagy, E.

AU - Leiter, E.

AU - Pócsi, I.

AU - Hagen, Silke

AU - Meyer, Vera

AU - Csernoch, L.

PY - 2006/7

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